Machine-Learning-Guided Engineering of an NADH-Dependent 7ß-Hydroxysteroid Dehydrogenase for Economic Synthesis of Ursodeoxycholic Acid.

Wang, Mu-Qiang; You, Zhi-Neng; Yang, Bing-Yi; Xia, Zi-Wei; Chen, Qi; Pan, Jiang; Li, Chun-Xiu; Xu, Jian-He

Wang, Mu-Qiang; You, Zhi-Neng; Yang, Bing-Yi; Xia, Zi-Wei; Chen, Qi; Pan, Jiang; Li, Chun-Xiu; Xu, Jian-He.

Afiliação

Wang MQ; Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, P. R. China.
You ZN; Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, P. R. China.
Yang BY; Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, P. R. China.
Xia ZW; Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, P. R. China.
Chen Q; Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, P. R. China.
Pan J; Shanghai Collaborative Innovation Center for Biomanufacturing, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, P. R. China.
Li CX; Laboratory of Biocatalysis and Synthetic Biotechnology, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, P. R. China.
Xu JH; Shanghai Collaborative Innovation Center for Biomanufacturing, School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, P. R. China.

J Agric Food Chem ; 71(49): 19672-19681, 2023 Dec 13.

Article em En | MEDLINE | ID: mdl-38016669

Assuntos

NAD; Ácido Ursodesoxicólico; Ácido Ursodesoxicólico/metabolismo; NADP/metabolismo; Teorema de Bayes; Hidroxiesteroide Desidrogenases/genética; Hidroxiesteroide Desidrogenases/metabolismo

Palavras-chave

7ß-hydroxysteroid dehydrogenase; NADH dependency; biotransformation; machine learning; protein engineering; ursodeoxycholic acid

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ácido Ursodesoxicólico / NAD Idioma: En Revista: J Agric Food Chem Ano de publicação: 2023 Tipo de documento: Article País de publicação: Estados Unidos

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