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Impact of coagulation on SARS-CoV-2 and PMMoV viral signal in wastewater solids.
Hegazy, Nada; Tian, Xin; D'Aoust, Patrick M; Pisharody, Lakshmi; Towhid, Syeda Tasneem; Mercier, Élisabeth; Zhang, Zhihao; Wan, Shen; Thakali, Ocean; Kabir, Md Pervez; Fang, Wanting; Nguyen, Tram B; Ramsay, Nathan T; MacKenzie, Alex E; Graber, Tyson E; Guilherme, Stéphanie; Delatolla, Robert.
Afiliação
  • Hegazy N; Department of Civil Engineering, University of Ottawa, Ottawa, ON, Canada.
  • Tian X; Department of Civil Engineering, University of Ottawa, Ottawa, ON, Canada.
  • D'Aoust PM; Department of Civil Engineering, University of Ottawa, Ottawa, ON, Canada.
  • Pisharody L; Department of Civil Engineering, University of Ottawa, Ottawa, ON, Canada.
  • Towhid ST; Department of Civil Engineering, University of Ottawa, Ottawa, ON, Canada.
  • Mercier É; Department of Civil Engineering, University of Ottawa, Ottawa, ON, Canada.
  • Zhang Z; Department of Civil Engineering, University of Ottawa, Ottawa, ON, Canada.
  • Wan S; Department of Civil Engineering, University of Ottawa, Ottawa, ON, Canada.
  • Thakali O; Department of Civil Engineering, University of Ottawa, Ottawa, ON, Canada.
  • Kabir MP; Department of Civil Engineering, University of Ottawa, Ottawa, ON, Canada.
  • Fang W; Department of Civil Engineering, University of Ottawa, Ottawa, ON, Canada.
  • Nguyen TB; Department of Civil Engineering, University of Ottawa, Ottawa, ON, Canada.
  • Ramsay NT; Department of Civil Engineering, University of Ottawa, Ottawa, ON, Canada.
  • MacKenzie AE; Children's Hospital of Eastern Ontario Research Institute, Ottawa, ON, Canada.
  • Graber TE; Children's Hospital of Eastern Ontario Research Institute, Ottawa, ON, Canada.
  • Guilherme S; Department of Civil Engineering, University of Ottawa, Ottawa, ON, Canada.
  • Delatolla R; Department of Civil Engineering, University of Ottawa, Ottawa, ON, Canada. Robert.Delatolla@uOttawa.ca.
Environ Sci Pollut Res Int ; 31(4): 5242-5253, 2024 Jan.
Article em En | MEDLINE | ID: mdl-38112868
ABSTRACT
Wastewater surveillance (WWS) of SARS-CoV-2 has become a crucial tool for monitoring COVID-19 cases and outbreaks. Previous studies have indicated that SARS-CoV-2 RNA measurement from testing solid-rich primary sludge yields better sensitivity compared to testing wastewater influent. Furthermore, measurement of pepper mild mottle virus (PMMoV) signal in wastewater allows for precise normalization of SARS-CoV-2 viral signal based on solid content, enhancing disease prevalence tracking. However, despite the widespread adoption of WWS, a knowledge gap remains regarding the impact of ferric sulfate coagulation, commonly used in enhanced primary clarification, the initial stage of wastewater treatment where solids are sedimented and removed, on SARS-CoV-2 and PMMoV quantification in wastewater-based epidemiology. This study examines the effects of ferric sulfate addition, along with the associated pH reduction, on the measurement of SARS-CoV-2 and PMMoV viral measurements in wastewater primary clarified sludge through jar testing. Results show that the addition of Fe3+ concentrations in the conventional 0 to 60 mg/L range caused no effect on SARS-CoV-2 N1 and N2 gene region measurements in wastewater solids. However, elevated Fe3+ concentrations were shown to be associated with a statistically significant increase in PMMoV viral measurements in wastewater solids, which consequently resulted in the underestimation of PMMoV-normalized SARS-CoV-2 viral signal measurements (N1 and N2 copies/copies of PMMoV). The observed pH reduction from coagulant addition did not contribute to the increased PMMoV measurements, suggesting that this phenomenon arises from the partitioning of PMMoV viral particles into wastewater solids.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Compostos Férricos / Tobamovirus / Águas Residuárias / COVID-19 Limite: Humans Idioma: En Revista: Environ Sci Pollut Res Int Assunto da revista: SAUDE AMBIENTAL / TOXICOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Canadá
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Compostos Férricos / Tobamovirus / Águas Residuárias / COVID-19 Limite: Humans Idioma: En Revista: Environ Sci Pollut Res Int Assunto da revista: SAUDE AMBIENTAL / TOXICOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Canadá