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Improvement in Yield of Extracellular Vesicles Derived from Edelweiss Callus Treated with LED Light and Enhancement of Skin Anti-Aging Indicators.
Kim, Mi-Jung; Ko, Hoon; Kim, Ji-Young; Kim, Hye-Jin; Kim, Hwi-Yeob; Cho, Hang-Eui; Cho, Hyun-Dae; Seo, Won-Sang; Kang, Hee-Cheol.
Afiliação
  • Kim MJ; Human & Microbiome Communicating Laboratory, GFC Co., Ltd., Hwaseong 18471, Republic of Korea.
  • Ko H; Creative Innovation Research Center, Cosmecca Korea Co., Ltd., Seongnam 13488, Republic of Korea.
  • Kim JY; Human & Microbiome Communicating Laboratory, GFC Co., Ltd., Hwaseong 18471, Republic of Korea.
  • Kim HJ; Human & Microbiome Communicating Laboratory, GFC Co., Ltd., Hwaseong 18471, Republic of Korea.
  • Kim HY; Creative Innovation Research Center, Cosmecca Korea Co., Ltd., Seongnam 13488, Republic of Korea.
  • Cho HE; Creative Innovation Research Center, Cosmecca Korea Co., Ltd., Seongnam 13488, Republic of Korea.
  • Cho HD; Creative Innovation Research Center, Cosmecca Korea Co., Ltd., Seongnam 13488, Republic of Korea.
  • Seo WS; Human & Microbiome Communicating Laboratory, GFC Co., Ltd., Hwaseong 18471, Republic of Korea.
  • Kang HC; Human & Microbiome Communicating Laboratory, GFC Co., Ltd., Hwaseong 18471, Republic of Korea.
Curr Issues Mol Biol ; 45(12): 10159-10178, 2023 Dec 16.
Article em En | MEDLINE | ID: mdl-38132480
ABSTRACT
The process of skin aging is currently recognized as a disease, and extracellular vesicles (EVs) are being used to care for it. While various EVs are present in the market, there is a growing need for research on improving skin conditions through microbial and plant-derived EVs. Edelweiss is a medicinal plant and is currently an endangered species. Callus culture is a method used to protect rare medicinal plants, and recently, research on EVs using callus culture has been underway. In this study, the researchers used LED light to increase the productivity of Edelweiss EVs and confirmed that productivity was enhanced by LED exposure. Additionally, improvements in skin anti-aging indicators were observed. Notably, M-LED significantly elevated callus fresh and dry weight, with a DW/FW ratio of 4.11%, indicating enhanced proliferation. Furthermore, M-LED boosted secondary metabolite production, including a 20% increase in total flavonoids and phenolics. The study explores the influence of M-LED on EV production, revealing a 2.6-fold increase in concentration compared to darkness. This effect is consistent across different plant species (Centella asiatica, Panax ginseng), demonstrating the universality of the phenomenon. M-LED-treated EVs exhibit a concentration-dependent inhibition of reactive oxygen species (ROS) production, surpassing dark-cultured EVs. Extracellular melanin content analysis reveals M-LED-cultured EVs' efficacy in reducing melanin production. Additionally, the expression of key skin proteins (FLG, AQP3, COL1) is significantly higher in fibroblasts treated with M-LED-cultured EVs. These results are expected to provide valuable insights into research on improving the productivity of plant-derived EVs and enhancing skin treatment using plant-derived EVs.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Curr Issues Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Curr Issues Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2023 Tipo de documento: Article