Simultaneous extraction and quantitative analysis of S-Methyl-l-Cysteine Sulfoxide, sulforaphane and glucosinolates in cruciferous vegetables by liquid chromatography mass spectrometry.
Food Chem X
; 21: 101065, 2024 Mar 30.
Article
em En
| MEDLINE
| ID: mdl-38187949
ABSTRACT
Sulfur containing compounds including glucosinolates (GLS), sulforaphane (SFN) and S-methyl-l-cysteine sulfoxide (SMCSO) have been proposed to be partly responsible for the beneficial health effects of cruciferous vegetables. As such, greater understanding of their measurements within foods is important to estimate intake in humans and to inform dietary intervention studies. Herein is described a simple and sensitive method for simultaneous analysis of 20 GLS, SFN and SMCSO by liquid chromatography mass spectrometry. Analytes were effectively retained and resolved on an Xbridge C18 column. Detection can be achieved using high resolution or unit resolution mass spectrometry; the latter making the method more applicable to large studies. Quantitative analysis using calibration standards was demonstrated for 10 GLS, SFN and SMCSO. A further 10 GLS were tentatively identified using high resolution mass spectrometry. The use of surrogate GLS standards was shown to be unreliable, with closely related GLS displaying significantly different ionisation efficiencies.
4-methoxyglucobrassicin (PubChem CID: 656563); Brassica; Cruciferous; Glucobrassicanapin (PubChem CID: 5485207); Glucobrassicin (PubChem CID: 5484743); Glucoerucin (PubChem CID: 656539); Glucoiberin (PubChem CID: 954862); Gluconapin (PubChem CID: 9548620); Gluconasturtiin (PubChem CID: 656555); Glucoraphanin (PubChem CID: 9548634); Glucosinolates; LC-MS; Progoitrin (PubChem CID: 5281139); S-methyl cysteine sulfoxide: (PubChem CID: 82142).; Sinigrin (PubChem CID: 23682211); Sulforaphane, S-methyl-l-cysteine sulfoxide
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Food Chem X
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Austrália