Development of Murine Anterior Interbody and Posterolateral Spinal Fusion Techniques.
J Bone Joint Surg Am
; 106(8): 735-745, 2024 Apr 17.
Article
em En
| MEDLINE
| ID: mdl-38194481
ABSTRACT
BACKGROUND:
Multiple animal models have previously been utilized to investigate anterior fusion techniques, but a mouse model has yet to be developed. The purpose of this study was to develop murine anterior interbody and posterolateral fusion techniques.METHODS:
Mice underwent either anterior interbody or posterolateral spinal fusion. A protocol was developed for both procedures, including a description of the relevant anatomy. Samples were subjected to micro-computed tomography to assess fusion success and underwent biomechanical testing with use of 4-point bending. Lastly, samples were fixed and embedded for histologic evaluation.RESULTS:
Surgical techniques for anterior interbody and posterolateral fusion were developed. The fusion rate was 83.3% in the anterior interbody model and 100% in the posterolateral model. Compared with a control, the posterolateral model exhibited a greater elastic modulus. Histologic analysis demonstrated endochondral ossification between bridging segments, further confirming the fusion efficacy in both models.CONCLUSIONS:
The murine anterior interbody and posterolateral fusion models are efficacious and provide an ideal platform for studying the molecular and cellular mechanisms mediating spinal fusion. CLINICAL RELEVANCE Given the extensive genetic tools available in murine disease models, use of fusion models such as ours can enable determination of the underlying genetic pathways involved in spinal fusion.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fusão Vertebral
/
Vértebras Lombares
Tipo de estudo:
Guideline
Limite:
Animals
Idioma:
En
Revista:
J Bone Joint Surg Am
Ano de publicação:
2024
Tipo de documento:
Article