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Designer protein compartments for microbial metabolic engineering.
Fang, Zhen; Zhu, Ya-Jiao; Qian, Zhi-Gang; Xia, Xiao-Xia.
Afiliação
  • Fang Z; State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, People's Republic of China.
  • Zhu YJ; State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, People's Republic of China.
  • Qian ZG; State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, People's Republic of China. Electronic address: zgqian@sjtu.
  • Xia XX; State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, People's Republic of China. Electronic address: xiaoxiaxia@s
Curr Opin Biotechnol ; 85: 103062, 2024 02.
Article em En | MEDLINE | ID: mdl-38199036
ABSTRACT
Protein compartments are distinct structures assembled in living cells via self-assembly or phase separation of specific proteins. Significant efforts have been made to discover their molecular structures and formation mechanisms, as well as their fundamental roles in spatiotemporal control of cellular metabolism. Here, we review the design and construction of synthetic protein compartments for spatial organization of target metabolic pathways toward increased efficiency and specificity. In particular, we highlight the compartmentalization strategies and recent examples to speed up desirable metabolic reactions, to reduce the accumulation of toxic metabolic intermediates, and to switch competing metabolic pathways. We also identify the most important challenges that need to be addressed for exploitation of these designer compartments as a versatile toolkit in metabolic reprogramming.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Redes e Vias Metabólicas / Engenharia Metabólica Idioma: En Revista: Curr Opin Biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Redes e Vias Metabólicas / Engenharia Metabólica Idioma: En Revista: Curr Opin Biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de publicação: Reino Unido