A green strategy for collagen extraction from tannery raw trimmings using papain enzyme: Process optimization by MW-TOPSIS for enhanced yield.

ABSTRACT

The leather industry poses a significant environmental problem through the extensive discharge of trimming waste, primarily composed of skin matrix rich in proteins. Developing a green approach for utilizing this waste can contribute to the sustainable recovery of proteins, transforming them into valuable bioresources. This study introduces an environmentally friendly and economically viable approach to extract collagen from tannery raw trimming waste using papain enzyme-derived from papaya leaves. The research involved extensive assessments and trials to optimize the enzymatic hydrolysis process. The highest collagen recovery was achieved by hydrolyzing 5 % (w/v) delimed powder with 4 % (w/v) crude papain enzyme from papaya leaf powder, maintaining it at 60 °C for 6 h and at pH 5. Collagen extraction from raw trimming waste using acetic acid was also performed, with the optimized papain enzyme-based hydrolysis process resulting in approximately 91 % yield, while conventional acetic acid method yielded approximately 84 %. To evaluate the performance of the enzymatic hydrolysis process in comparison to acid hydrolysis and hydrothermal hydrolysis, an integrated MW-TOPSIS framework was proposed. This framework determined that enzymatic hydrolysis achieved the highest closeness coefficient value (Ri = 0.40), indicating its superiority as the preferred alternative among the tested methods.