Impact of the Different Chemical-Based Decellularization Protocols on the Properties of the Caprine Pericardium.
Cardiovasc Eng Technol
; 15(3): 279-289, 2024 Jun.
Article
em En
| MEDLINE
| ID: mdl-38347340
ABSTRACT
PURPOSE:
This study aims to decellularized caprine pericardium tissue with varied non-ionic surfactant and anionic detergent concentrations.METHODS:
Protocol A consists of 1%, 0.5%, and 0.25% (w/v) sodium dodecyl sulphate (SDS). Protocol B uses 1%, 0.5%, and 0.25% (w/v) Triton X-100. Protocol C comprised 0.5% SDS + 0.5% Triton X-100, 0.5% + 0.25%, and 0.25% SDS + 0.5% Triton X-100.RESULTS:
Protocol B left a few countable cells in the pericardium tissue, but treatments A and C removed all cells. DNA quantification also demonstrated that protocol B had the most leftover DNA after decellularization. The pericardium tissue treated with an equal combination of anionic detergent and non-ionic surfactant preserves the matrix. However, changing the anionic detergent-non-ionic surfactant ratio disrupted the microstructure. Protocol A decreased pericardium tissue secant modulus (p < 0.05). Protocol B-treated pericardium tissue matched native tissue secant modulus and ultimate tensile stress. Protocol C strengthened pericardium tissue.CONCLUSION:
The intact extracellular matrix and biomechanical properties like native tissues require optimal chemical doses and combinations.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pericárdio
/
Dodecilsulfato de Sódio
/
Cabras
/
Octoxinol
Tipo de estudo:
Guideline
Limite:
Animals
Idioma:
En
Revista:
Cardiovasc Eng Technol
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Índia