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scMicrobe PTA: Near Complete Genomes from Single Bacterial Cells.
Bowers, Robert M; Gonzalez-Pena, Veronica; Wardhani, Kartika; Goudeau, Danielle; Blow, Matthew James; Udwary, Daniel; Klein, David; Vill, Albert C; Brito, Ilana L; Woyke, Tanja; Malmstrom, Rex; Gawad, Charles.
Afiliação
  • Bowers RM; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Gonzalez-Pena V; Department of Pediatrics, Stanford University, Stanford, CA, USA.
  • Wardhani K; Department of Pediatrics, Stanford University, Stanford, CA, USA.
  • Goudeau D; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Blow MJ; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Udwary D; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Klein D; Department of Pediatrics, Stanford University, Stanford, CA, USA.
  • Vill AC; Chan Zuckerberg Biohub, San Francisco, CA, USA.
  • Brito IL; Chan Zuckerberg Biohub, San Francisco, CA, USA.
  • Woyke T; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Malmstrom R; DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
  • Gawad C; Department of Pediatrics, Stanford University, Stanford, CA, USA.
bioRxiv ; 2024 Jan 31.
Article em En | MEDLINE | ID: mdl-38352480
ABSTRACT
Microbial genomes produced by single-cell amplification are largely incomplete. Here, we show that primary template amplification (PTA), a novel single-cell amplification technique, generated nearly complete genomes from three bacterial isolate species. Furthermore, taxonomically diverse genomes recovered from aquatic and soil microbiomes using PTA had a median completeness of 81%, whereas genomes from standard amplification approaches were usually <30% complete. PTA-derived genomes also included more associated viruses and biosynthetic gene clusters.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: BioRxiv Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: BioRxiv Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos