Formation of an instantaneous nick for highly efficient adenylation of oligonucleotides by ligase without subsequent jointing.
Chem Commun (Camb)
; 60(21): 2942-2945, 2024 Mar 07.
Article
em En
| MEDLINE
| ID: mdl-38374791
ABSTRACT
By forming a nick at the adenylation site instantaneously, nucleic acids are efficiently adenylated by T4 DNA ligase. The subsequent ligation is successfully suppressed in terms of rapid conversion of the instantaneous nick to a more stable gap. It is helpful to understand enzymatic ligation dynamics, and the adenylated products can be used for various practical applications.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligonucleotídeos
/
Ligases
Idioma:
En
Revista:
Chem Commun (Camb)
Assunto da revista:
QUIMICA
Ano de publicação:
2024
Tipo de documento:
Article
País de publicação:
Reino Unido