Highly Sensitive and Rapid Screening Technique for the Detection of Organophosphate Pesticides and Copper Compounds Using Bifunctional Recombinant TrxA-PvCarE1.
J Agric Food Chem
; 72(9): 5003-5013, 2024 Mar 06.
Article
em En
| MEDLINE
| ID: mdl-38408326
ABSTRACT
Enabling the detection of organophosphate pesticide (OP) residues through enzyme inhibition-based technology is crucial for ensuring food safety and human health. However, the use of acetylcholinesterase, the primary target enzyme for OPs, isolated from animals in practical production poses challenges in terms of sensitivity and batch stability. To address this issue, we identified a highly sensitive and reproducible biorecognition element, TrxA-PvCarE1, derived from red kidney beans and successfully overexpressed it in Escherichia coli. The resulting recombinant TrxA-PvCarE1 exhibited remarkable sensitivity toward 10 OPs, surpassing that of commercial acetylcholinesterase. Additionally, this approach demonstrated the capability to simultaneously detect copper compounds with high sensitivity, expanding the range of pesticides detectable using the traditional enzyme inhibition method. Spiking recovery tests conducted on cowpea and carrot samples verified the suitability of the TrxA-PvCarE1-based technique for real-life sample analysis. In summary, this study highlights a promising comprehensive candidate for the rapid detection of pesticide residues.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Praguicidas
/
Resíduos de Praguicidas
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Técnicas Biossensoriais
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Inseticidas
Limite:
Animals
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Humans
Idioma:
En
Revista:
J Agric Food Chem
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China