Effect of gamma-radiation on fowl sperm function in vitro and in vivo.
J Reprod Fertil
; 75(2): 617-22, 1985 Nov.
Article
em En
| MEDLINE
| ID: mdl-3840843
ABSTRACT
Doses of up to 300 Gy of ionizing radiation had little effect on fowl sperm morphology, ATP content and motility when measured in vitro. Fertility of eggs from hens inseminated with spermatozoa receiving 50 Gy, in terms of post-oviducal development, was less than 4%. However, 35% appeared 'fertile' by macroscopic examination of the germinal disc of unincubated eggs. These contained few embryonic cells, although the vitelline membrane contained many trapped spermatozoa. After doses of 100 Gy or more, inseminated spermatozoa were not found in the vitelline membrane and no fertile or apparently fertile eggs were produced; nor did such spermatozoa enter the utero-vaginal sperm-host glands. Genetic transformation using fowl spermatozoa irradiated with doses in excess of 100 Gy appears to be an unlikely prospect.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espermatozoides
Limite:
Animals
Idioma:
En
Revista:
J Reprod Fertil
Ano de publicação:
1985
Tipo de documento:
Article