Harnessing HetHydrogel: A Universal Platform to Dropletize Single-Cell Multiomics.
Small Methods
; 8(7): e2301631, 2024 Jul.
Article
em En
| MEDLINE
| ID: mdl-38419597
ABSTRACT
A universal platform is developed for dropletizing single cell plate-based multiomic assays, consisting of three main pillars a miniaturized open Heterogeneous Hydrogel reactor (abbreviated HetHydrogel) for multi-step biochemistry, its tunable permeability that allows Tn5 tagmentation, and single cell droplet barcoding. Through optimizing the HetHydrogel manufacturing procedure, the chemical composition, and cell permeation conditions, simultaneous high-throughput mitochondrial DNA genotyping and chromatin profiling at the single-cell level are demonstrated using a mixed-species experiment. This platform offers a powerful way to investigate the genotype-phenotype relationships of various mtDNA mutations in biological processes. The HetHydrogel platform is believed to have the potential to democratize droplet technologies, upgrading a whole range of plate-based single cell assays to high throughput format.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA Mitocondrial
/
Hidrogéis
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Análise de Célula Única
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Small Methods
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China