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Gut microbiota dynamics and fecal SCFAs after colonoscopy: accelerating microbiome stabilization by Clostridium butyricum.
Chen, Zhenhui; Yu, Lu; Liu, Jiaxin; Kong, Jingjing; Deng, Xiaoshi; Guo, Xiaotong; Shan, Jiamin; Zhou, Daixuan; Li, Wendan; Lin, Yangfan; Huang, Wanwen; Zeng, Weisen; Shi, Xinlong; Bai, Yang; Fan, Hongying.
Afiliação
  • Chen Z; Department of Microbiology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, China.
  • Yu L; Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Liu J; Department of Microbiology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, China.
  • Kong J; Department of Microbiology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, China.
  • Deng X; Department of Microbiology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, China.
  • Guo X; Department of Microbiology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, China.
  • Shan J; Department of Microbiology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, China.
  • Zhou D; Guangdong Provincial Key Laboratory of Gastroenterology, Inst. Of Gastroenterology of Guangdong Province, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Li W; Department of Microbiology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, China.
  • Lin Y; Guangdong Provincial Key Laboratory of Gastroenterology, Inst. Of Gastroenterology of Guangdong Province, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, China.
  • Huang W; Department of Microbiology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, China.
  • Zeng W; Department of Cell Biology, School of Basic Medicine, Southern Medical University, Guangzhou, China.
  • Shi X; Department of Colorectal Surgery, Gansu Provincial Hospital, Lanzhou, China.
  • Bai Y; Guangdong Provincial Key Laboratory of Gastroenterology, Inst. Of Gastroenterology of Guangdong Province, Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, China. baiyang1030@hotmail.com.
  • Fan H; Department of Microbiology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, China. gzfhy@smu.edu.cn.
J Transl Med ; 22(1): 222, 2024 03 01.
Article em En | MEDLINE | ID: mdl-38429821
ABSTRACT

BACKGROUND:

Colonoscopy is a classic diagnostic method with possible complications including abdominal pain and diarrhoea. In this study, gut microbiota dynamics and related metabolic products during and after colonoscopy were explored to accelerate gut microbiome balance through probiotics.

METHODS:

The gut microbiota and fecal short-chain fatty acids (SCFAs) were analyzed in four healthy subjects before and after colonoscopy, along with seven individuals supplemented with Clostridium butyricum. We employed 16S rRNA sequencing and GC-MS to investigate these changes. We also conducted bioinformatic analysis to explore the buk gene, encoding butyrate kinase, across C. butyricum strains from the human gut.

RESULTS:

The gut microbiota and fecal short-chain fatty acids (SCFAs) of four healthy subjects were recovered on the 7th day after colonoscopy. We found that Clostridium and other bacteria might have efficient butyric acid production through bioinformatic analysis of the buk and assessment of the transcriptional level of the buk. Supplementation of seven healthy subjects with Clostridium butyricum after colonoscopy resulted in a quicker recovery and stabilization of gut microbiota and fecal SCFAs on the third day.

CONCLUSION:

We suggest that supplementation of Clostridium butyricum after colonoscopy should be considered in future routine clinical practice.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Clostridium butyricum / Microbiota / Microbioma Gastrointestinal Limite: Humans Idioma: En Revista: J Transl Med Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Clostridium butyricum / Microbiota / Microbioma Gastrointestinal Limite: Humans Idioma: En Revista: J Transl Med Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Reino Unido