[Analytical Method for Melengestrol Acetate in Livestock Products Using LC-MS/MS].
Shokuhin Eiseigaku Zasshi
; 65(1): 15-19, 2024.
Article
em Ja
| MEDLINE
| ID: mdl-38432897
ABSTRACT
The present study verified that it is possible to analyze melengesterol acetate using the existing multi-residue method. Melengestrol acetate was extracted from livestock products using acidic acetonitrile acidified with acetic acid in the presence of n-hexane and anhydrous sodium sulfate. The crude extracts were cleaned up using an octadecylsilanized silica gel cartridge column. Separation by HPLC was performed using an octadecylsilanized silica gel column with linear gradient elution of 0.1 vol% formic acid and acetonitrile containing 0.1 vol% formic acid. For the determination of the analyte, tandem mass spectrometry with positive ion electrospray ionization was used. In recovery tests using four livestock products fortified with maximum residue limits levels of melengestrol acetate (0.001-0.02 mg/kg), the truenesses ranged from 82% to 100%, and the repeatabilities for the entire procedure ranged from 0.5 RSD% to 5.6 RSD%. In recovery tests using 11 livestock products fortified with 0.0005 mg/kg of melengestrol acetate, the truenesses ranged from 88% to 99%, and the repeatabilities ranged from 1.3 RSD% to 5.4 RSD%. The limit of quantification for melengestrol acetate in livestock products was 0.0005 mg/kg.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Formiatos
/
Espectrometria de Massa com Cromatografia Líquida
/
Acetato de Melengestrol
Limite:
Animals
Idioma:
Ja
Revista:
Shokuhin Eiseigaku Zasshi
Ano de publicação:
2024
Tipo de documento:
Article
País de publicação:
Japão