A novel nanoliposome model platform mimicking SARS-CoV-2 as a bioreceptor to dissect the amperometric response in biosensor applications.
Int J Biol Macromol
; 264(Pt 1): 130530, 2024 Apr.
Article
em En
| MEDLINE
| ID: mdl-38437936
ABSTRACT
In this study, we proposed to investigate the response of an electrochemical-based immunosensor via nanoliposomes carrying the SARS-CoV-2 Spike-S1 protein. In this regard, we prepared RNA encapsulated nanoliposome functionalized with a specific SARS-CoV-2 Spike-S1 protein as a SARS-CoV-2 model. Then, this new nanoliposome mimicking SARS-CoV-2 was used as the bio-recognizing agent of an immunosensor developed to detect the SARS-CoV-2 within the scope of the study. The working electrode of the immunosensor was coated with chitosan polymer, decorated with SARS-CoV-2 Spike antibody, to achieve antibody-antigen matching on the electrode surface. SARS-CoV-2 mimicking nanoliposomes at various concentrations was used to achieve an amperometric response and the analytical parameters of the sensor were calculated from the relationship between the immunosensor's current values depending on the number of these matches with regard to varying antigen concentrations. Linear measurement range, LOD and measurement sensitivity were calculated as 53 pM-8 nM, 3.79 pM and 55.47 µA nM-1 cm-2, respectively. The standard deviation of the same measurements in the developed immunosensor was 0.33 %.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
COVID-19
Limite:
Humans
Idioma:
En
Revista:
Int J Biol Macromol
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Turquia
País de publicação:
Holanda