Synthesis of Cross-Linked and Sterilized Water-Soluble Electrospun Nanofiber Biomatrix of Streptomycin-Imbedded PVA/CHN/ß-CD for Wound Healing.

ABSTRACT

The diagnosis and prognosis of chronic wounds are demanding and require objective assessment. Because of their potential medicinal applications, the syntheses of biopolymeric chitosan (CHN) structure and PVA-based mixed electrospun nanofibers with biomimetic features were thoroughly investigated. This study created different formulas, including a guest molecule and capping agent, using supporting PVA as a vehicle. CHN was used as a biomodifier, and beta-cyclodextrin (ß-CD) as a smoother and more efficiently entraps streptomycin (STP) compared with the silver sheet wound dressing. The relevant analyses showed that the size distribution increased with the incorporation of PVA, CHN, and ß-CD to 120.3, 161.9, and 192.02 nm. The webs boosted particle size and released content stability to 96.4% without compromising the nanofiber structure. Examining the synergistic effects of the PVA/CHN/STP/ß-CD nanoformulation against pathogenic strains of S. aureus, P. aeruginosa, and Aspergillus niger, clean zones were 47 ± 3.4, 45 ± 3.0, and 49 ± 3.7 mm were produced. PVA/CHN/STP/ß-CD formula exhibited a 98.9 ± 0.6% cell viability and wound closure of 100% at 72 h. The results reveal that the PVA/CHN/STP/ß-CD formula is promising for medical applications, especially in wound healing, compared with the silver sheet.