In vitro Effect of EBV Infection on HERV-K18 env Expression in the Presence and Absence of Vitamin D in Multiple Sclerosis Patients.
Iran Biomed J
; 2023 Oct 29.
Article
em En
| MEDLINE
| ID: mdl-38468371
ABSTRACT
Background:
HERV-K env is associated with several neurological disorders, including MS. Clinical studies have demonstrated a plausible interaction between HERV-K env and other MS risk factors. The present study aimed to investigate the possible association between HERV-K18 env and TGF-ß. We further assessed the in vitro effect of EBV infection on HERV-K18 env expression in the presence and absence of vitamin D in MS patients.Methods:
PBMCs from 20 MS patients and 20 healthy controls were infected with the B95.8 EBV, seeded into 24-well plates and incubated in the presence or absence of 100 nM of 1,25(OH)D3. The expression levels of HERV-K18 env and TGF-ß were measured using real-time PCR.Results:
While the expression level of HERV-K18 env was significantly higher in MS patients than the healthy controls, this trend for TGF-ß was significantly reverse. Interestingly, an inverse correlation was found between HERV-K18 env and TGF-ß expression in MS patients, although the in vitro stimulation of PBMCs with EBV and vitamin D showed no significant differences in terms of HERV-K18 expression.Conclusion:
Our findings highlight the potential role of HERV-K18 env in MS patients.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Iran Biomed J
Assunto da revista:
BIOLOGIA
/
MEDICINA
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Irã
País de publicação:
Irã