Accumulation of docosapentaenoic acid (n-3 DPA) in a novel isolate of the marine ichthyosporean Sphaeroforma arctica.
Biotechnol Lett
; 46(3): 373-383, 2024 Jun.
Article
em En
| MEDLINE
| ID: mdl-38493279
ABSTRACT
OBJECTIVE:
Currently, there is lack of a consistent and highly enriched source for docosapentaenoic acid (n-3 DPA, C225), and this work report the isolation of microorganism that naturally produces n-3 DPA.RESULTS:
In this work, we screened microorganisms in our culture collections with the goal to isolate a strain with high levels of n-3 DPA. We isolated a strain of Sphaeroforma arctica that produces up to 11% n-3 DPA in total fatty acid and has a high n-3 DPA to DHA/EPA ratio. The cell growth of the isolated strain was characterized using microscopy imaging and flow cytometer technologies to confirm the coenocytic pattern of cell divisions previously described in S. arctica. Our novel isolate of S. arctica grew more robustly and produced significantly more n-3 DPA compared to previously isolated and described strains indicating the uniqueness of the discovered strain.CONCLUSION:
Overall, this work reports a first isolate n-3 DPA producing microorganism and establishes the foundation for future strain improvement and elucidation of the physiological function of this LC-PUFA for human nutrition and health.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácidos Graxos Insaturados
Idioma:
En
Revista:
Biotechnol Lett
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Estados Unidos