Experiment to Demonstrate Pesticide-Induced Antimicrobial Resistance (AMR): An Emerging Health Threat.

ABSTRACT

Background Pesticides, including insecticides, herbicides, and fungicides, are essential for global food production, boosting crop yields, and preventing disease transmission. However, their excessive and improper use raises concerns about potential long-term consequences, affecting microbial ecosystems and fostering antimicrobial resistance. Materials and methods The objective of the study was to identify the effect of the pesticide compound (Imidacloprid 17.1% w/w) on the ATCC Escherichia coli. An experiment was conducted on ATCC Escherichia coli 27852. A 0.5 McFarland suspension of the strain was incubated in the presence of a pesticide compound (Imidacloprid 17.1% w/w) at a dilution of 14, 18, and 116. at 370C. Antibiotic susceptibility for cefoxitin, ciprofloxacin, ceftazidime, amikacin, and imipenem was determined via the Kirby-Bauer disk diffusion test at intervals of 24 hours, 48 hours, seven days, and 21 days. The results were then compared to the standard zone of inhibition diameter for ATCC Escherichia coli 27852 by Clinical and Laboratory Standards Institute (CLSI) guidelines. Results No bacterial growth was detected at pesticide dilutions of 11 and 12, indicating their inability to tolerate high pesticide concentrations. However, growth became evident at a 14 dilution and beyond, with mutants thriving within the inhibition zone. The experiment caused significant alterations in the inhibition zone sizes for all antibiotics, especially notable with imipenem, amikacin, and ceftazidime compared to the initial zone size for ATCC Escherichia coli 27852. Conclusion Our study concludes that the pesticide (Imidacloprid 17.1% w/w) significantly influences antibiotic resistance, especially with carbapenems, aminoglycosides, and cephalosporins in the tested groups at various concentrations and durations of exposure.