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Constitutive Overexpression of an NB-ARC Gene from Wild Chinese Vitis quinquangularis in Arabidopsis thaliana Enhances Resistance to Phytopathogenic Oomycete and Bacteria.
Yin, Xiangjing; Zha, Qian; Sun, Pengpeng; Xi, Xiaojun; Jiang, Aili.
Afiliação
  • Yin X; Forest and Fruit Tree Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
  • Zha Q; Shanghai Key Labs of Protected Horticultural Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
  • Sun P; Forest and Fruit Tree Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
  • Xi X; Shanghai Key Labs of Protected Horticultural Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
  • Jiang A; Forest and Fruit Tree Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
Int J Mol Sci ; 25(6)2024 Mar 12.
Article em En | MEDLINE | ID: mdl-38542196
ABSTRACT
Resistance (R) genes were used to recognize pathogen effectors directly or indirectly in plants and activate defense signal pathways. Most of these R proteins consist of a nucleotide-binding adaptor (NB-ARC) domain, a leucine-rich repeat (LRR) domain and some also have a coiled-coil (CC) structure. In this study, we cloned a gene which encodes the CC-NB-ARC-LRR R protein (VqCNL) from Chinese wild grapevine Vitis. quinquangularis accession 'Dan-2'. The transcript of VqCNL was obviously induced by inoculation with Plasmopara viticola and the salicylic acid (SA) treatment. The results of sequence analysis showed that the VqCNL gene contained a CC domain at the N-terminus, along with an NB-ARC and an LRR domain at the C-terminus. We transferred this gene into wildtype Arabidopsis and treated transgenic lines with Hyaloperonospora arabidopsidis (Hpa) and Pseudomonas syringae pv. tomato DC3000 (Pst DC3000); the results demonstrated that VqCNL promotes broad spectrum resistance to pathogens. Furthermore, qPCR analysis displayed that VqCNL may display a significant function in disease resistance via activating SA signaling pathways. In general, these conclusions primarily demonstrated that VqCNL enhances the disease resistance level in plants and contributes to future research of the R gene identification for grape breeding biotechnology.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oomicetos / Arabidopsis / Vitis / Proteínas de Arabidopsis País/Região como assunto: Asia Idioma: En Revista: Int J Mol Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oomicetos / Arabidopsis / Vitis / Proteínas de Arabidopsis País/Região como assunto: Asia Idioma: En Revista: Int J Mol Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China