SARS-CoV-2 mutant spectra as variant of concern nurseries: endless variation?
Front Microbiol
; 15: 1358258, 2024.
Article
em En
| MEDLINE
| ID: mdl-38559344
ABSTRACT
Introduction:
SARS-CoV-2 isolates of a given clade may contain low frequency genomes that encode amino acids or deletions which are typical of a different clade.Methods:
Here we use high resolution ultra-deep sequencing to analyze SARS-CoV-2 mutant spectra.Results:
In 6 out of 11 SARS-CoV-2 isolates from COVID-19 patients, the mutant spectrum of the spike (S)-coding region included two or more amino acids or deletions, that correspond to discordant viral clades. A similar observation is reported for laboratory populations of SARS-CoV-2 USA-WA1/2020, following a cell culture infection in the presence of remdesivir, ribavirin or their combinations. Moreover, some of the clade-discordant genome residues are found in the same haplotype within an amplicon.Discussion:
We evaluate possible interpretations of these findings, and reviewed precedents for rapid selection of genomes with multiple mutations in RNA viruses. These considerations suggest that intra-host evolution may be sufficient to generate minority sequences which are closely related to sequences typical of other clades. The results provide a model for the origin of variants of concern during epidemic spreadâin particular Omicron lineagesâthat does not require prolonged infection, involvement of immunocompromised individuals, or participation of intermediate, non-human hosts.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Front Microbiol
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Espanha
País de publicação:
Suíça