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Dietary fiber guar gum-induced shift in gut microbiota metabolism and intestinal immune activity enhances susceptibility to colonic inflammation.
Paudel, Devendra; Nair, Divek V T; Tian, Sangshan; Hao, Fuhua; Goand, Umesh K; Joseph, Grace; Prodes, Eleni; Chai, Zhi; Robert, Chloé E M; Chassaing, Benoit; Patterson, Andrew D; Singh, Vishal.
Afiliação
  • Paudel D; Department of Nutritional Sciences, The Pennsylvania State University, University Park, PA, USA.
  • Nair DVT; Department of Nutritional Sciences, The Pennsylvania State University, University Park, PA, USA.
  • Tian S; Department of Nutritional Sciences, The Pennsylvania State University, University Park, PA, USA.
  • Hao F; Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA, USA.
  • Goand UK; Department of Nutritional Sciences, The Pennsylvania State University, University Park, PA, USA.
  • Joseph G; Department of Nutritional Sciences, The Pennsylvania State University, University Park, PA, USA.
  • Prodes E; Department of Nutritional Sciences, The Pennsylvania State University, University Park, PA, USA.
  • Chai Z; Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Robert CEM; INSERM U1016, team "Mucosal microbiota in chronic inflammatory diseases", CNRS UMR 8104, Université Paris Cité, Paris, France.
  • Chassaing B; INSERM U1306, Microbiome-Host Interaction group, Institut Pasteur, Université Paris Cité, Paris, France.
  • Patterson AD; INSERM U1016, team "Mucosal microbiota in chronic inflammatory diseases", CNRS UMR 8104, Université Paris Cité, Paris, France.
  • Singh V; INSERM U1306, Microbiome-Host Interaction group, Institut Pasteur, Université Paris Cité, Paris, France.
Gut Microbes ; 16(1): 2341457, 2024.
Article em En | MEDLINE | ID: mdl-38630030
ABSTRACT
With an increasing interest in dietary fibers (DFs) to promote intestinal health and the growth of beneficial gut bacteria, there is a continued rise in the incorporation of refined DFs in processed foods. It is still unclear how refined fibers, such as guar gum, affect the gut microbiota activity and pathogenesis of inflammatory bowel disease (IBD). Our study elucidated the effect and underlying mechanisms of guar gum, a fermentable DF (FDF) commonly present in a wide range of processed foods, on colitis development. We report that guar gum containing diet (GuD) increased the susceptibility to colonic inflammation. Specifically, GuD-fed group exhibited severe colitis upon dextran sulfate sodium (DSS) administration, as evidenced by reduced body weight, diarrhea, rectal bleeding, and shortening of colon length compared to cellulose-fed control mice. Elevated levels of pro-inflammatory markers in both serum [serum amyloid A (SAA), lipocalin 2 (Lcn2)] and colon (Lcn2) and extensive disruption of colonic architecture further affirmed that GuD-fed group exhibited more severe colitis than control group upon DSS intervention. Amelioration of colitis in GuD-fed group pre-treated with antibiotics suggest a vital role of intestinal microbiota in GuD-mediated exacerbation of intestinal inflammation. Gut microbiota composition and metabolite analysis in fecal and cecal contents, respectively, revealed that guar gum primarily enriches Actinobacteriota, specifically Bifidobacterium. Guar gum also altered multiple genera belonging to phyla Bacteroidota and Firmicutes. Such shift in gut microbiota composition favored luminal accumulation of intermediary metabolites succinate and lactate in the GuD-fed mice. Colonic IL-18 and tight junction markers were also decreased in the GuD-fed group. Importantly, GuD-fed mice pre-treated with recombinant IL-18 displayed attenuated colitis. Collectively, unfavorable changes in gut microbiota activity leading to luminal accumulation of lactate and succinate, reduced colonic IL-18, and compromised gut barrier function following guar gum feeding contributed to increased colitis susceptibility.
Guar gum increased susceptibility to colitisGuar gum-induced exacerbation of colitis is gut microbiota dependentGuar gum-induced shift in microbiota composition favored the accumulation of luminal intermediate metabolites succinate and lactateGuar gum-fed mice exhibited reduced colonic level of IL-18 and tight junction molecules.Exogenous IL-18 administration partly rescued mice from guar gum-induced colitis susceptibility.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Colite / Gomas Vegetais / Microbioma Gastrointestinal / Galactanos / Mananas Limite: Animals Idioma: En Revista: Gut Microbes Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Colite / Gomas Vegetais / Microbioma Gastrointestinal / Galactanos / Mananas Limite: Animals Idioma: En Revista: Gut Microbes Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos
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