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Exploring the mechanism underlying the antifungal activity of chitosan-based ZnO, CuO, and SiO2 nanocomposites as nanopesticides against Fusarium solani and Alternaria solani.
Krumova, Ekaterina; Benkova, Dayana; Stoyancheva, Galina; Dishliyska, Vladislava; Miteva-Staleva, Jeny; Kostadinova, Aneliya; Ivanov, Kamen; El-Sayed, Kh; Staneva, Galya; Elshoky, Hisham A.
Afiliação
  • Krumova E; Institute of Microbiology, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria. Electronic address: e_krumova@microbio.bas.bg.
  • Benkova D; Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria.
  • Stoyancheva G; Institute of Microbiology, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria.
  • Dishliyska V; Institute of Microbiology, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria.
  • Miteva-Staleva J; Institute of Microbiology, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria.
  • Kostadinova A; Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria. Electronic address: aneliakk@gmail.com.
  • Ivanov K; Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria; Institute of Electronics, Bulgarian Academy of Sciences, 1784 Sofia, Bulgaria.
  • El-Sayed K; Faculty of Engineering, Galala University, Attaka 51745, Suez, Egypt; Nanotechnology and Advanced Materials Central Lab, Agricultural Research Center, Giza 12619, Egypt; Regional Center for Food and Feed, Agricultural Research Center, Giza 12619, Egypt.
  • Staneva G; Institute of Biophysics and Biomedical Engineering, Bulgarian Academy of Sciences, Sofia 1113, Bulgaria. Electronic address: gstaneva@bio21.bas.bg.
  • Elshoky HA; Nanotechnology and Advanced Materials Central Lab, Agricultural Research Center, Giza 12619, Egypt; Regional Center for Food and Feed, Agricultural Research Center, Giza 12619, Egypt; Tumor Biology Research Program, Department of Research, Children's Cancer Hospital, Cairo 11441, Egypt. Electronic a
Int J Biol Macromol ; 268(Pt 1): 131702, 2024 May.
Article em En | MEDLINE | ID: mdl-38643917
ABSTRACT
Chitosan-based nanocomposites (CS NCs) are gaining considerable attention as multifaceted antifungal agents. This study investigated the antifungal activity of NCs against two phytopathogenic strains Fusarium solani (F. solani) and Alternaria solani (A. solani). Moreover, it sheds light on their underlying mechanisms of action. The NCs, CS-ZnO, CS-CuO, and CS-SiO2, were characterized using advanced methods. Dynamic and electrophoretic light scattering techniques revealed their size range (60-170 nm) and cationic nature, as indicated by the positive zeta potential values (from +16 to +22 mV). Transmission electron microscopy revealed the morphology of the NCs as agglomerates formed between the chitosan and oxide components. X-ray diffraction patterns confirmed crystalline structures with specific peaks indicating their constituents. Antifungal assessments using the agar diffusion technique demonstrated significant inhibitory effects of the NCs on both fungal strains (1.5 to 4-fold), surpassing the performance of the positive control, nystatin. Notably, the NCs exhibited superior antifungal potency, with CS-ZnO NCs being the most effective. A. solani was the most sensitive strain to the studied agents. Furthermore, the tested NCs induced oxidative stress in fungal cells, which elevated stress biomarker levels, such as superoxide dismutase (SOD) activity and protein carbonyl content (PCC), 2.5 and 6-fold for the most active CS-CuO in F. solani respectively. Additionally, they triggered membrane lipid peroxidation up to 3-fold higher compared to control, a process that potentially compromises membrane integrity. Laurdan fluorescence spectroscopy highlighted alterations in the molecular organization of fungal cell membranes induced by the NCs. CS-CuO NCs induced a membrane rigidifying effect, while CS-SiO2 and CS-ZnO could rigidify membranes in A. solani and fluidize them in F. solani. In summary, this study provides an in-depth understanding of the interactions of CS-based NCs with two fungal strains, showing their antifungal activity and offering insights into their mechanisms of action. These findings emphasize the potential of these NCs as effective and versatile antifungal agents.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óxido de Zinco / Dióxido de Silício / Cobre / Quitosana / Nanocompostos / Alternaria / Fusarium / Antifúngicos Idioma: En Revista: Int J Biol Macromol Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óxido de Zinco / Dióxido de Silício / Cobre / Quitosana / Nanocompostos / Alternaria / Fusarium / Antifúngicos Idioma: En Revista: Int J Biol Macromol Ano de publicação: 2024 Tipo de documento: Article