Sortase-Modified Cholera Toxoids Show Specific Golgi Localization.
Toxins (Basel)
; 16(4)2024 Apr 16.
Article
em En
| MEDLINE
| ID: mdl-38668619
ABSTRACT
Cholera toxoid is an established tool for use in cellular tracing in neuroscience and cell biology. We use a sortase labeling approach to generate site-specific N-terminally modified variants of both the A2-B5 heterohexamer and B5 pentamer forms of the toxoid. Both forms of the toxoid are endocytosed by GM1-positive mammalian cells, and while the heterohexameric toxoid was principally localized in the ER, the B5 pentamer showed an unexpectedly specific localization in the medial/trans-Golgi. This study suggests a future role for specifically labeled cholera toxoids in live-cell imaging beyond their current applications in neuronal tracing and labeling of lipid rafts in fixed cells.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cisteína Endopeptidases
/
Toxina da Cólera
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Complexo de Golgi
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Toxins (Basel)
Ano de publicação:
2024
Tipo de documento:
Article
País de publicação:
Suíça