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Comprehensive assessment of the UV-filter 2-ethylhexyl salicylate and its phase I/II metabolites in urine by extended enzymatic hydrolysis and on-line SPE LC-MS/MS.
Kuhlmann, Laura; Hiller, Julia; Göen, Thomas.
Afiliação
  • Kuhlmann L; Institute and Outpatient Clinic of Occupational, Social, and Environmental Medicine, Friedrich-Alexander-Universität Erlangen-Nürnberg, Henkestr. 9-11, 91054, Erlangen, Germany.
  • Hiller J; Institute and Outpatient Clinic of Occupational, Social, and Environmental Medicine, Friedrich-Alexander-Universität Erlangen-Nürnberg, Henkestr. 9-11, 91054, Erlangen, Germany.
  • Göen T; Institute and Outpatient Clinic of Occupational, Social, and Environmental Medicine, Friedrich-Alexander-Universität Erlangen-Nürnberg, Henkestr. 9-11, 91054, Erlangen, Germany. Electronic address: thomas.goeen@fau.de.
Talanta ; 276: 126223, 2024 Aug 15.
Article em En | MEDLINE | ID: mdl-38728806
ABSTRACT
2-ethylhexyl salicylate (EHS) is used as a UV filter in personal-care products, such as sunscreen, to prevent skin damage through UV radiation. The application of EHS-containing products leads to systemic EHS absorption, metabolization and excretion. To measure EHS and its corresponding metabolite levels in urine, a comprehensive analytical procedure based on an extended enzymatic hydrolysis, on-line-SPE, and UPLC-MS/MS was developed. The method covers a large profile of seven metabolites (including isomeric structures) as well as EHS itself in a run time only of 18 min. Easy sample preparation, consisting of a 2-h hydrolysis step, followed by on-line enrichment and purification, add to the efficiency of the method. An update, compared to a previous method for the determination of EHS and metabolites in urine, is that, during hydrolysis, both glucuronide and sulfate conjugates are considered. The method was furthermore applied to urine samples after a real-life exposure scenario to EHS-containing sunscreen. The method is highly sensitive with limits of detection ranging from 6 to 65 ng/L. Moreover, it is characterized by good precision data, accuracy, and robustness to matrix influences. Application of the method to urine samples following dermal exposure to an EHS-containing sunscreen revealed EHS as the main biomarker after dermal exposure, followed by the major biomarkers 5OH-EHS, 5cx-EPS, 4OH-EHS and 5oxo-EHS. The expansion and optimization of this method decisively contributes to the research on the dermal metabolism of EHS and can be applied in exposure studies and for human biomonitoring.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Protetores Solares / Salicilatos / Extração em Fase Sólida Limite: Humans Idioma: En Revista: Talanta Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Alemanha País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Protetores Solares / Salicilatos / Extração em Fase Sólida Limite: Humans Idioma: En Revista: Talanta Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Alemanha País de publicação: Holanda