Functional mouse hepatocytes derived from interspecies chimeric livers effectively mitigate chronic liver fibrosis.
Stem Cell Reports
; 19(6): 877-889, 2024 Jun 11.
Article
em En
| MEDLINE
| ID: mdl-38729156
ABSTRACT
Liver disease is a major global health challenge. There is a shortage of liver donors worldwide, and hepatocyte transplantation (HT) may be an effective treatment to overcome this problem. However, the present approaches for generation of hepatocytes are associated with challenges, and interspecies chimera-derived hepatocytes produced by interspecies blastocyst complementation (IBC) may be promising donor hepatocytes because of their more comprehensive hepatic functions. In this study, we isolated mouse hepatocytes from mouse-rat chimeric livers using IBC and found that interspecies chimera-derived hepatocytes exhibited mature hepatic functions in terms of lipid accumulation, glycogen storage, and urea synthesis. Meanwhile, they were more similar to endogenous hepatocytes than hepatocytes derived in vitro. Interspecies chimera-derived hepatocytes could relieve chronic liver fibrosis and reside in the injured liver after transplantation. Our results suggest that interspecies chimera-derived hepatocytes are a potentially reliable source of hepatocytes and can be applied as a therapeutic approach for HT.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Quimera
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Hepatócitos
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Fígado
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Cirrose Hepática
Limite:
Animals
Idioma:
En
Revista:
Stem Cell Reports
Ano de publicação:
2024
Tipo de documento:
Article