Successful Extension of Vascularized Composite Allograft Perfusion Cold Storage to 24 h in a Rat Hindlimb Transplant Model.
Transplant Direct
; 10(6): e1623, 2024 Jun.
Article
em En
| MEDLINE
| ID: mdl-38757052
ABSTRACT
Background:
Vascularized composite allograft transplantation is a treatment option for complex tissue injuries; however, ischemia reperfusion injury and high acute rejection rates remain a challenge. Hypothermic machine perfusion using acellular storage perfusate is a potential solution. This study evaluated the University of Wisconsin Kidney Preservation Solution-1 (KPS-1) compared with normal saline (NS) for preservation of donor rat hindlimbs subjected to 24 h of ex vivo perfusion cold storage.Methods:
Hindlimbs were subjected to 24-h perfusion cold storage with heparinized KPS-1 (nâ =â 6) or heparinized NS (nâ =â 6). Flow, resistance, and pH were measured continuously. At the end of the 24-h period, tissue was collected for histological analysis of edema and apoptosis.Results:
KPS-1 perfused limbs showed significantly less edema than the NS group, as evidenced by lower limb weight gain (Pâ <â 0.001) and less interfascicular space (Pâ <â 0.001). KPS-perfused muscle had significantly less cell death than NS-perfused muscle based on terminal deoxynucleotidyl transferase dUTP nick-end labeling (Pâ <â 0.001) and cleaved caspase-3 staining (Pâ =â 0.045). During hypothermic machine perfusion, a significant decrease in pH over time was detected in both groups, with a significantly greater decline in pH in the KPS-1 group than in the NS group. There were no significant differences overall and over time in flow rate or vascular resistance between the KPS and NS groups.Conclusions:
Perfusion with KPS-1 can successfully extend vascularized composite allograft perfusion cold storage for 24 h in a rat hindlimb model without significant edema or cell death.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Transplant Direct
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Colômbia