Protocol to culture enteric glial cells from the submucosal and myenteric plexi of neonatal and juvenile pig colons.
STAR Protoc
; 5(2): 103057, 2024 Jun 21.
Article
em En
| MEDLINE
| ID: mdl-38762883
ABSTRACT
Here, we present our protocol to culture enteric glial cells from the submucosal and myenteric plexus of neonatal and juvenile pig colons. We describe steps for colon isolation, microdissection, and enzymatic and mechanical dissociation. We include procedures for passaging and analyzing cell yield, freeze/thaw efficiency, and purity. This protocol allows for the generation of primary cultures of enteric glial cells from single-cell suspensions of microdissected layers of the colon wall and can be used to culture enteric glia from human colon specimens. For complete details on the use and execution of this protocol, please refer to Ziegler et al.1.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neuroglia
/
Colo
/
Técnicas de Cultura de Células
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Animais Recém-Nascidos
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Plexo Mientérico
Limite:
Animals
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Estados Unidos