A series of spontaneously blinking dyes for super-resolution microscopy.

Holland, Katie L; Plutkis, Sarah E; Daugird, Timothy A; Sau, Abhishek; Grimm, Jonathan B; English, Brian P; Zheng, Qinsi; Dave, Sandeep; Rahman, Fariha; Xie, Liangqi; Dong, Peng; Tkachuk, Ariana N; Brown, Timothy A; Singer, Robert H; Liu, Zhe; Galbraith, Catherine G; Musser, Siegfried M; Legant, Wesley R; Lavis, Luke D

Holland, Katie L; Plutkis, Sarah E; Daugird, Timothy A; Sau, Abhishek; Grimm, Jonathan B; English, Brian P; Zheng, Qinsi; Dave, Sandeep; Rahman, Fariha; Xie, Liangqi; Dong, Peng; Tkachuk, Ariana N; Brown, Timothy A; Singer, Robert H; Liu, Zhe; Galbraith, Catherine G; Musser, Siegfried M; Legant, Wesley R; Lavis, Luke D.

Afiliação

Holland KL; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Plutkis SE; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Daugird TA; Department of Pharmacology, University of North Carolina-Chapel Hill Medical School, Chapel Hill, NC, USA.
Sau A; Department of Cell Biology and Genetics, Texas A&M University, School of Medicine, College Station, TX, USA.
Grimm JB; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
English BP; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Zheng Q; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Dave S; Department of Cell Biology and Genetics, Texas A&M University, School of Medicine, College Station, TX, USA.
Rahman F; Joint Department of Biomedical Engineering, University of North Carolina-Chapel Hill and North Carolina State University, Chapel Hill, NC, USA.
Xie L; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Dong P; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Tkachuk AN; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Brown TA; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Singer RH; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Liu Z; Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York, USA.
Galbraith CG; Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA.
Musser SM; Department of Biomedical Engineering and Knight Cancer Institute, Oregon Health and Science University, Portland, OR, USA.
Legant WR; Department of Cell Biology and Genetics, Texas A&M University, School of Medicine, College Station, TX, USA.
Lavis LD; Department of Pharmacology, University of North Carolina-Chapel Hill Medical School, Chapel Hill, NC, USA.

bioRxiv ; 2024 Feb 24.

Article em En | MEDLINE | ID: mdl-38766149

ABSTRACT

ABSTRACT

Spontaneously blinking fluorophores permit the detection and localization of individual molecules without reducing buffers or caging groups, thus simplifying single-molecule localization microscopy (SMLM). The intrinsic blinking properties of such dyes are dictated by molecular structure and modulated by environment, which can limit utility. We report a series of tuned spontaneously blinking dyes with duty cycles that span two orders of magnitude, allowing facile SMLM in cells and dense biomolecular structures.

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: BioRxiv Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: EEUU / ESTADOS UNIDOS / ESTADOS UNIDOS DA AMERICA / EUA / UNITED STATES / UNITED STATES OF AMERICA / US / USA

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