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Adjuvant-induced arthritis promotes vascular hyporesponsiveness to phenylephrine through a nitric oxide-related mechanism.
Araujo, T S; Spadella, M A; Carlos, C P; Tirapelli, C R; Chagas, E F B; Pinheiro, J C D; Chies, A B.
Afiliação
  • Araujo TS; Laboratório de Farmacologia, Faculdade de Medicina de Marília, Marília, SP, Brasil.
  • Spadella MA; Laboratório de Embriologia Humana, Faculdade de Medicina de Marília, Marília, SP, Brasil.
  • Carlos CP; Laboratório de Pesquisa Experimental, Faculdade de Medicina Faceres, São José do Rio Preto, SP, Brasil.
  • Tirapelli CR; Disciplina de Fisiologia, Faculdade de Medicina de Marília, Marília, SP, Brasil.
  • Chagas EFB; Laboratório de Farmacologia Cardiovascular, Escola de Enfermagem de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil.
  • Pinheiro JCD; Centro Interdisciplinar de Diabetes, Universidade de Marília, Marília, SP, Brasil.
  • Chies AB; Programa de Mestrado Interdisciplinar em Interações Estruturais e Funcionais em Reabilitação, Universidade de Marília, Marília, SP, Brasil.
Braz J Med Biol Res ; 57: e13304, 2024.
Article em En | MEDLINE | ID: mdl-38775546
ABSTRACT
Arthritis has important cardiovascular repercussions. Phenylephrine-induced vasoconstriction is impaired in rat aortas in the early phase of the adjuvant-induced arthritis (AIA), around the 15th day post-induction. Therefore, the present study aimed to verify the effects of AIA on hyporesponsiveness to phenylephrine in rat aortas. AIA was induced by intradermal injection of Mycobacterium tuberculosis (3.8 mg/dL) in the right hind paw of male Wistar rats (n=27). Functional experiments in isolated aortas were carried out 15 days after AIA induction. Morphometric and stereological analyses of the aortas were also performed 36 days after the induction of AIA. AIA did not promote structural modifications in the aortas at any of the time points studied. AIA reduced phenylephrine-induced contraction in endothelium-intact aortas, but not in endothelium-denuded aortas. However, AIA did not change KCl-induced contraction in either endothelium-intact or denuded aortas. L-NAME (non-selective NOS inhibitor), 1400W (selective iNOS inhibitor), and ODQ (guanylyl cyclase inhibitor) reversed AIA-induced hyporesponsiveness to phenylephrine in intact aortas. 7-NI (selective nNOS inhibitor) increased the contraction induced by phenylephrine in aortas from AIA rats. In summary, the hyporesponsiveness to phenylephrine induced by AIA was endothelium-dependent and mediated by iNOS-derived NO through activation of the NO-guanylyl cyclase pathway.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fenilefrina / Artrite Experimental / Ratos Wistar / Óxido Nítrico Limite: Animals Idioma: En Revista: Braz J Med Biol Res Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fenilefrina / Artrite Experimental / Ratos Wistar / Óxido Nítrico Limite: Animals Idioma: En Revista: Braz J Med Biol Res Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil