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DNA Vaccine Encoding a Modified Hemagglutinin Trimer of Avian Influenza A Virus H5N8 Protects Mice from Viral Challenge.
Litvinova, Victoria R; Rudometov, Andrey P; Rudometova, Nadezhda B; Kisakov, Denis N; Borgoyakova, Mariya B; Kisakova, Lyubov A; Starostina, Ekaterina V; Fando, Anastasia A; Yakovlev, Vladimir A; Tigeeva, Elena V; Ivanova, Ksenia I; Gudymo, Andrei S; Ilyicheva, Tatiana N; Marchenko, Vasiliy Yu; Sergeev, Artemiy A; Ilyichev, Alexander A; Karpenko, Larisa I.
Afiliação
  • Litvinova VR; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Rudometov AP; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Rudometova NB; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Kisakov DN; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Borgoyakova MB; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Kisakova LA; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Starostina EV; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Fando AA; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Yakovlev VA; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Tigeeva EV; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Ivanova KI; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Gudymo AS; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Ilyicheva TN; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Marchenko VY; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Sergeev AA; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Ilyichev AA; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
  • Karpenko LI; Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector¼, Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, Russia.
Vaccines (Basel) ; 12(5)2024 May 14.
Article em En | MEDLINE | ID: mdl-38793789
ABSTRACT
The development of a safe and effective vaccine against avian influenza A virus (AIV) H5N8 is relevant due to the widespread distribution of this virus in the bird population and the existing potential risk of human infection, which can lead to significant public health concerns. Here, we developed an experimental pVAX-H5 DNA vaccine encoding a modified trimer of AIV H5N8 hemagglutinin. Immunization of BALB/c mice with pVAX-H5 using jet injection elicited high titer antibody response (the average titer in ELISA was 1 × 105), and generated a high level of neutralizing antibodies against H5N8 and T-cell response, as determined by ELISpot analysis. Both liquid and lyophilized forms of pVAX-H5 DNA vaccine provided 100% protection of immunized mice against lethal challenge with influenza A virus A/turkey/Stavropol/320-01/2020 (H5N8). The results obtained indicate that pVAX-H5 has good opportunities as a vaccine candidate against the influenza A virus (H5N8).
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Vaccines (Basel) Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Federação Russa
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Vaccines (Basel) Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Federação Russa