Evaluation of different molecular systems for detection and quantification of SARS-CoV-2 RNA from wastewater samples.
J Virol Methods
; 328: 114956, 2024 Jul.
Article
em En
| MEDLINE
| ID: mdl-38796134
ABSTRACT
Wastewater-based epidemiology has proved to be a suitable approach for tracking the spread of epidemic agents including SARS-CoV-2 RNA. Different protocols have been developed for quantitative detection of SARS-CoV-2 RNA from wastewater samples, but little is known on their performance. In this study we compared three protocols based on Reverse Transcription Real Time-PCR (RT-PCR) and one based on Droplet Digital PCR (ddPCR) for SARS-CoV-2 RNA detection from 35 wastewater samples. Overall, SARS-CoV-2 RNA was detected by at least one method in 85.7â¯% of samples, while 51.4â¯%, 22.8â¯% and 8.6â¯% resulted positive with two, three or all four methods, respectively. Protocols based on commercial RT-PCR assays and on Droplet Digital PCR showed an overall higher sensitivity vs. an in-house assay. The use of more than one system, targeting different genes, could be helpful to increase detection sensitivity.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
RNA Viral
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Sensibilidade e Especificidade
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Águas Residuárias
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SARS-CoV-2
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COVID-19
Limite:
Humans
Idioma:
En
Revista:
J Virol Methods
Ano de publicação:
2024
Tipo de documento:
Article