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Poplar glutathione S-transferase PtrGSTF8 contributes to reactive oxygen species scavenging and salt tolerance.
Song, Yushuang; Yu, Keji; Zhang, Shuwen; Li, Yi; Xu, Changwen; Qian, Hongping; Cui, Yaning; Guo, Yayu; Zhang, Xi; Li, Ruili; Dixon, Richard A; Lin, Jinxing.
Afiliação
  • Song Y; College of Biological Sciences & Biotechnology, Beijing Forestry University, Beijing, 100083, China; Institute of Tree Development and Genome Editing, Beijing Forestry University, Beijing, 100083, China.
  • Yu K; College of Biological Sciences & Biotechnology, Beijing Forestry University, Beijing, 100083, China; Institute of Tree Development and Genome Editing, Beijing Forestry University, Beijing, 100083, China.
  • Zhang S; College of Biological Sciences & Biotechnology, Beijing Forestry University, Beijing, 100083, China.
  • Li Y; College of Biological Sciences & Biotechnology, Beijing Forestry University, Beijing, 100083, China; Institute of Tree Development and Genome Editing, Beijing Forestry University, Beijing, 100083, China.
  • Xu C; College of Biological Sciences & Biotechnology, Beijing Forestry University, Beijing, 100083, China; Institute of Tree Development and Genome Editing, Beijing Forestry University, Beijing, 100083, China.
  • Qian H; College of Biological Sciences & Biotechnology, Beijing Forestry University, Beijing, 100083, China; Institute of Tree Development and Genome Editing, Beijing Forestry University, Beijing, 100083, China.
  • Cui Y; College of Biological Sciences & Biotechnology, Beijing Forestry University, Beijing, 100083, China; Institute of Tree Development and Genome Editing, Beijing Forestry University, Beijing, 100083, China.
  • Guo Y; College of Biological Sciences & Biotechnology, Beijing Forestry University, Beijing, 100083, China; Institute of Tree Development and Genome Editing, Beijing Forestry University, Beijing, 100083, China.
  • Zhang X; College of Biological Sciences & Biotechnology, Beijing Forestry University, Beijing, 100083, China; Institute of Tree Development and Genome Editing, Beijing Forestry University, Beijing, 100083, China.
  • Li R; College of Biological Sciences & Biotechnology, Beijing Forestry University, Beijing, 100083, China; Institute of Tree Development and Genome Editing, Beijing Forestry University, Beijing, 100083, China.
  • Dixon RA; BioDiscovery Institute and Department of Biological Sciences, University of North Texas, Denton, TX, 76203, USA.
  • Lin J; College of Biological Sciences & Biotechnology, Beijing Forestry University, Beijing, 100083, China; Institute of Tree Development and Genome Editing, Beijing Forestry University, Beijing, 100083, China. Electronic address: linjx@ibcas.ac.cn.
Plant Physiol Biochem ; 212: 108766, 2024 Jul.
Article em En | MEDLINE | ID: mdl-38797011
ABSTRACT
Glutathione S-transferases (GSTs) constitute a protein superfamily encoded by a large gene family and play a crucial role in plant growth and development. However, their precise functions in wood plant responses to abiotic stress are not fully understood. In this study, we isolated a Phi class glutathione S-transferase-encoding gene, PtrGSTF8, from poplar (Populus alba × P. glandulosa), which is significantly up-regulated under salt stress. Moreover, compared with wild-type (WT) plants, transgenic tobacco plants exhibited significant salt stress tolerance. Under salt stress, PtrGSTF8-overexpressing tobacco plants showed a significant increase in plant height and root length, and less accumulation of reactive oxygen species. In addition, these transgenic tobacco plants exhibited higher superoxide dismutase, peroxidase, and catalase activities and reduced malondialdehyde content compared with WT plants. Quantitative real-time PCR experiments showed that the overexpression of PtrGSTF8 increased the expression of numerous genes related to salt stress. Furthermore, PtrMYB108, a MYB transcription factor involved in salt resistance in poplar, was found to directly activate the promoter of PtrGSTF8, as demonstrated by yeast one-hybrid assays and luciferase complementation assays. Taken together, these findings suggest that poplar PtrGSTF8 contributes to enhanced salt tolerance and confers multiple growth advantages when overexpressed in tobacco.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Nicotiana / Plantas Geneticamente Modificadas / Espécies Reativas de Oxigênio / Populus / Tolerância ao Sal / Glutationa Transferase Idioma: En Revista: Plant Physiol Biochem Assunto da revista: BIOQUIMICA / BOTANICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Plantas / Nicotiana / Plantas Geneticamente Modificadas / Espécies Reativas de Oxigênio / Populus / Tolerância ao Sal / Glutationa Transferase Idioma: En Revista: Plant Physiol Biochem Assunto da revista: BIOQUIMICA / BOTANICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China