Your browser doesn't support javascript.
loading
Cryo-electron tomography reveals the packaging pattern of RuBisCOs in Synechococcus ß-carboxysome.
Kong, Wen-Wen; Zhu, Yun; Zhao, Heng-Rui; Du, Kang; Zhou, Rui-Qian; Li, Bo; Yang, Feng; Hou, Pu; Huang, Xia-He; Chen, Yuxing; Wang, Ying-Chun; Sun, Fei; Jiang, Yong-Liang; Zhou, Cong-Zhao.
Afiliação
  • Kong WW; School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China.
  • Zhu Y; National Key Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
  • Zhao HR; School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China.
  • Du K; School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China.
  • Zhou RQ; School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China.
  • Li B; School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China.
  • Yang F; School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China.
  • Hou P; School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China.
  • Huang XH; Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
  • Chen Y; School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China.
  • Wang YC; Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
  • Sun F; National Key Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: feisun@ibp.ac.cn.
  • Jiang YL; School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China. Electronic address: jyl@ustc.edu.cn.
  • Zhou CZ; School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China. Electronic address: zcz@ustc.edu.cn.
Structure ; 32(8): 1110-1120.e4, 2024 Aug 08.
Article em En | MEDLINE | ID: mdl-38823379
ABSTRACT
Carboxysomes are large self-assembled microcompartments that serve as the central machinery of a CO2-concentrating mechanism (CCM). Biogenesis of carboxysome requires the fine organization of thousands of individual proteins; however, the packaging pattern of internal RuBisCOs remains largely unknown. Here we purified the intact ß-carboxysomes from Synechococcus elongatus PCC 7942 and identified the protein components by mass spectrometry. Cryo-electron tomography combined with subtomogram averaging revealed the general organization pattern of internal RuBisCOs, in which the adjacent RuBisCOs are mainly arranged in three distinct manners head-to-head, head-to-side, and side-by-side. The RuBisCOs in the outermost layer are regularly aligned along the shell, the majority of which directly interact with the shell. Moreover, statistical analysis enabled us to propose an ideal packaging model of RuBisCOs in the ß-carboxysome. These results provide new insights into the biogenesis of ß-carboxysomes and also advance our understanding of the efficient carbon fixation functionality of carboxysomes.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ribulose-Bifosfato Carboxilase / Proteínas de Bactérias / Microscopia Crioeletrônica / Synechococcus / Tomografia com Microscopia Eletrônica Idioma: En Revista: Structure Assunto da revista: BIOLOGIA MOLECULAR / BIOQUIMICA / BIOTECNOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ribulose-Bifosfato Carboxilase / Proteínas de Bactérias / Microscopia Crioeletrônica / Synechococcus / Tomografia com Microscopia Eletrônica Idioma: En Revista: Structure Assunto da revista: BIOLOGIA MOLECULAR / BIOQUIMICA / BIOTECNOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos