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UBE2C as an Immune-Related Biomarker for Breast Cancer: A Study Based on Multiple Databases.
Cui, Yue; Wang, Hong-Zhi; Song, Ye; Yang, Shuang; Sai, Feng-Ying; Yu, De-Jun.
Afiliação
  • Cui Y; Central Laboratory of the Fifth Affiliated Hospital of Harbin Medical University, Daqing 163711, Heilongjian Province, China.
  • Wang HZ; Central Laboratory of the Fifth Affiliated Hospital of Harbin Medical University, Daqing 163711, Heilongjian Province, China.
  • Song Y; Central Laboratory of the Fifth Affiliated Hospital of Harbin Medical University, Daqing 163711, Heilongjian Province, China.
  • Yang S; Central Laboratory of the Fifth Affiliated Hospital of Harbin Medical University, Daqing 163711, Heilongjian Province, China.
  • Sai FY; Clinical Laboratory of The Fifth Affiliated Hospital of Harbin Medical University, Daqing 163316, Heilongjian Province, China.
  • Yu DJ; Clinical Laboratory of The Fifth Affiliated Hospital of Harbin Medical University, Daqing 163316, Heilongjian Province, China.
Chin Med Sci J ; 2024 May 20.
Article em En | MEDLINE | ID: mdl-38828693
ABSTRACT
Objective To screen the target gene UBE2C and explore its prognostic value and immune correlation in breast cancer (BRCA) using multiple databases. Methods The microarray expression datasets of BRCA were downloaded from the Gene Expresssion Omnibus database (GEO) and analyzed to obtain differentially expressed genes (DEGs). Hub genes were obtained by constructing and visualizing the protein-protein interaction network of DEGs. Then the key gene UBE2C was determined using R language, STRING, and Cytoscape, and the differential expression of UBE2C was verified using the external datasets, The Cancer Genome Atlas (TCGA) , and quantitative real-time PCR (qRT-PCR). The prognostic value and immunological correlation of UBE2C in BRCA were explored using R language, TIMER, and Gene Set Enrichment Analysis (GSEA).Results The expression of UBE2C was differentially upregulated in BRCA, as verified by TCGA and qRT-PCR. Prognostic analysis revealed that UBE2C served as an independent prognostic factor. High expression of UBE2C was associated with decreased immune infiltration levels of B cells, CD4+ T cells, CD8+ T cells, macrophages, and myeloid dendritic cells in BRCA tissue. The expression of UBE2C in BRCA showed a significant correlation with PDCD1, CD274, and CTLA4 expressions. There was a positive correlation between the expression of UBE2C and the tumor mutational burden and microsatellite instability. GSEA demonstrated that UBE2C expression significantly enriched 786 immune-related gene sets.Conclusions UBE2C expression in BRCA tissues can predict the survivals and prognosis of BRCA patients. Also, it is closely related to the BRCA immune microenvironment and can predict the effecacy of immunotherapy in BRCA patients. Therefore, UBE2C may be an potential immune-related prognostic biomarker for BRCA.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Chin Med Sci J Assunto da revista: TERAPIAS COMPLEMENTARES Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Chin Med Sci J Assunto da revista: TERAPIAS COMPLEMENTARES Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China