Comparison of Colistin Susceptibility via Two Different Methods in Gram-Negative Extensive Drug-Resistance Isolates from ICU Patients.
J Coll Physicians Surg Pak
; 34(6): 677-681, 2024 Jun.
Article
em En
| MEDLINE
| ID: mdl-38840350
ABSTRACT
OBJECTIVE:
To compare the susceptibility of colistin by two methods in extensive drug-resistant (XDR) Gram-negative isolates from ICU patients. STUDYDESIGN:
Cross-sectional comparative analysis. Place and Duration of the Study Department of Microbiology, Combined Military Hospital Karachi, Pakistan, from August 2022 to February 2023.METHODOLOGY:
A total of 100 clinical specimens received from the intensive care unit yielded growth of extensively drug-resistant gram-negative bacteria, which were evaluated for polymyxin E susceptibility. The agar dilution method was compared with the reference broth microdilution (BMD) method. Minimum inhibitory concentration (MIC) was noted for both methods.RESULTS:
Comparison of the MIC method by agar dilution showed a 90% correlation with the reference method of broth microdilution. With MICs within the acceptable range of the clinical and laboratory standards institute (CLSI) recommendations, 89 isolates were susceptible to colistin, whereas only 11 remained resistant. Polymyxin E's MIC 50 and MIC 90 were determined to be 1 and 2 µg/ml, respectively, with 97% susceptibility.CONCLUSION:
Agar dilution susceptibility method can be used for screening purposes for the susceptibility testing of polymyxin E. This method is reliable and can easily identify the heteroresistance. KEY WORDS Extensively drug-resistant, Broth microdilution, Multidrug-resistant, Agar dilution, Minimum inhibitory concentration, Colony forming unit.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Testes de Sensibilidade Microbiana
/
Infecções por Bactérias Gram-Negativas
/
Colistina
/
Farmacorresistência Bacteriana Múltipla
/
Bactérias Gram-Negativas
/
Unidades de Terapia Intensiva
/
Antibacterianos
Limite:
Humans
País/Região como assunto:
Asia
Idioma:
En
Revista:
J Coll Physicians Surg Pak
Assunto da revista:
MEDICINA
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Paquistão