Your browser doesn't support javascript.
loading
Effects of honokiol combined with resveratrol on bacteria responsible for oral malodor and their biofilm.
Zheng, Shiqian; Deng, Rongrong; Huang, Gengjiu; Ou, Zhiwen; Shen, Zhibin.
Afiliação
  • Zheng S; Research and Development Department (R&D), Guangdong Botanical Beauty Care Biotechnology Co. Ltd., Guangzhou, China.
  • Deng R; Research and Development Department (R&D), Guangdong Botanical Beauty Care Biotechnology Co. Ltd., Guangzhou, China.
  • Huang G; Research and Development Department (R&D), Guangdong Botanical Beauty Care Biotechnology Co. Ltd., Guangzhou, China.
  • Ou Z; Research and Development Department (R&D), Guangdong Botanical Beauty Care Biotechnology Co. Ltd., Guangzhou, China.
  • Shen Z; Research and Development Department (R&D), Guangdong Botanical Beauty Care Biotechnology Co. Ltd., Guangzhou, China.
J Oral Microbiol ; 16(1): 2361402, 2024.
Article em En | MEDLINE | ID: mdl-38860120
ABSTRACT

Background:

This study aimed to investigate the effect of honokiol combined with resveratrol on bacteria responsible for oral malodor and their biofilm.

Method:

This study investigated drug's MIC, FICI and dynamic bactericidal susceptibility activities against Pg and Fn. The effects of drugs on biofilm metabolic activity, biofilm total amount, and biofilm microstructure were determined by CCK-8 experiment, semi-quantitative adhesion experiment and SEM, respectively. The effects of drugs on biofilm genes, extracellular polysaccharides, proteins and DNA content were determined by qRT-PCR, phenol-sulfuric acid method, BCA method and Nano Drop one C, respectively.

Results:

The combination had synergistic antibacterial effect on Pg and Fn. 1/2×MIC and 1×MIC combination inhibit the whole process of Pg and Fn growth. The results showed that the combination effectively reduce biofilm metabolic activity and total amount, and destroy biofilm microstructure. The results showed that the combination downregulate the gene expression both Pg and Fn, reduce extracellular polysaccharides and DNA of Pg, and reduce extracellular proteins and DNA of Fn.

Conclusion:

This study showed that the combination had a synergistic antibacterial effect on Pg and Fn, reduced the biofilm extracellular matrix, inhibited biofilm formation, and downregulated the expression of genes related to biofilm formation.
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: J Oral Microbiol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: J Oral Microbiol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos