Spectrophotometric Assays for Measuring Hydroxypyruvate Reductase Activity.

Liepman, Aaron H; Jaworski, Mara; Ramirez-Lopez, Citlalxochilt

Liepman, Aaron H; Jaworski, Mara; Ramirez-Lopez, Citlalxochilt.

Afiliação

Liepman AH; Biology Department, Eastern Michigan University, Ypsilanti, MI, USA. aliepman@emich.edu.
Jaworski M; Biology Department, Eastern Michigan University, Ypsilanti, MI, USA.
Ramirez-Lopez C; Biology Department, Eastern Michigan University, Ypsilanti, MI, USA.

Methods Mol Biol ; 2792: 77-81, 2024.

Article em En | MEDLINE | ID: mdl-38861079

ABSTRACT

ABSTRACT

Hydroxypyruvate reductase (HPR; EC 1.1.1.81) activity is integral to the photorespiratory pathway. Within photorespiration, HPR catalyzes the reduction of hydroxypyruvate, a product of the serineglyoxylate aminotransferase reaction to glycerate, a substrate for glycerate kinase, using NADH as cofactor. Here we detail a spectrophotometric assay for measuring HPR activity in vitro by following the consumption of NADH at 340 nm.

Assuntos

Ensaios Enzimáticos; Hidroxipiruvato Redutase; Espectrofotometria; Espectrofotometria/métodos; Hidroxipiruvato Redutase/metabolismo; Ensaios Enzimáticos/métodos; NAD/metabolismo

Palavras-chave

Enzyme kinetics; Hydroxypyruvate reductase; NADH; Photorespiration; Spectrophotometric assay

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Espectrofotometria / Hidroxipiruvato Redutase / Ensaios Enzimáticos Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos

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