Spinal nerve ligation: An experimental model to study neuropathic pain in rats and mice.
Methods Cell Biol
; 188: 73-88, 2024.
Article
em En
| MEDLINE
| ID: mdl-38880529
ABSTRACT
Neuropathic pain, defined as the most terrible of all tortures, which a nerve wound may inflict, is a common chronic painful condition caused by gradual damage or dysfunction of the somatosensory nervous system. As with many chronic diseases, neuropathic pain has a profound economic and emotional impact worldwide and represents a major public health issue from a treatment standpoint. This condition involves multiple sensory symptoms including impaired transmission and perception of noxious stimuli, burning, shooting, spontaneous pain, mechanical or thermal allodynia and hyperalgesia. Current pharmacological options for the treatment of neuropathic pain are limited, ineffective and have unacceptable side effects. In this framework, a deeper understanding of the pathophysiology and molecular mechanisms associated with neuropathic pain is key to the development of promising new therapeutical approaches. For this purpose, a plethora of experimental models that mimic common clinical features of human neuropathic pain have been characterized in rodents, with the spinal nerve ligation (SNL) model being one of the most widely used. In this chapter, we provide a detailed surgical procedure of the SNL model used to induce neuropathic pain in rats and mice. We further describe the behavioral approaches used for stimulus-evoked and spontaneous pain assessment in rodents. Finally, we demonstrate that our SNL model induces multiple pain behaviors in rats and mice.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Nervos Espinhais
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Modelos Animais de Doenças
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Neuralgia
Limite:
Animals
Idioma:
En
Revista:
Methods Cell Biol
Ano de publicação:
2024
Tipo de documento:
Article