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Antibiotic Resistance in Enterococci and Enterobacteriaceae from Laboratory-Reared Fresh Mealworm Larvae (Tenebrio molitor L.) and Their Frass.
Turchi, Barbara; Mancini, Simone; Pedonese, Francesca; Resci, Ilaria; Torracca, Beatrice; Marconi, Francesca; Barone, Chiara; Nuvoloni, Roberta; Fratini, Filippo.
Afiliação
  • Turchi B; Department of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy.
  • Mancini S; Interdepartmental Center 'NUTRAFOOD', University of Pisa, Via del Borghetto 80, 56124 Pisa, Italy.
  • Pedonese F; Department of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy.
  • Resci I; Interdepartmental Center 'NUTRAFOOD', University of Pisa, Via del Borghetto 80, 56124 Pisa, Italy.
  • Torracca B; Department of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy.
  • Marconi F; Interdepartmental Center 'NUTRAFOOD', University of Pisa, Via del Borghetto 80, 56124 Pisa, Italy.
  • Barone C; Department of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy.
  • Nuvoloni R; Department of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy.
  • Fratini F; Department of Veterinary Sciences, University of Pisa, Viale delle Piagge 2, 56124 Pisa, Italy.
Pathogens ; 13(6)2024 May 28.
Article em En | MEDLINE | ID: mdl-38921754
ABSTRACT
The occurrence of antibiotic-resistant bacteria in foodstuff involves a human health risk. Edible insects are a precious resource; however, their consumption raises food safety issues. In this study, the occurrence of antibiotic resistant bacteria in laboratory-reared fresh mealworm larvae (Tenebrio molitor L.) and frass was assessed. Antibiotics were not used during the rearing. Enterobacteriaceae and enterococci were isolated from 17 larvae and eight frass samples. In total, 62 and 69 isolates presumed to belong to Enterobacteriaceae and Enterococcus spp., respectively, were obtained and tested for antibiotic susceptibility via disk diffusion. Based on the results, isolates were grouped, and representative resistant isolates were identified at species level through 16S rRNA gene sequencing. For enterococci resistance, percentages higher than 15% were observed for vancomycin and quinupristin-dalfopristin, whereas Enterobacteriaceae resistance higher than 25% was found against cefoxitin, ampicillin, and amoxicillin-clavulanic acid. Based on the species identification, the observed resistances seemed to be intrinsic both for enterococci and Enterobacteriaceae, except for some ß-lactams resistance in Shigella boydii (cefoxitin and aztreonam). These could be due to transferable genetic elements. This study suggests the need for further investigations to clarify the role of edible insects in the spreading of antibiotic resistance determinants through the food chain.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Pathogens Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Itália País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Pathogens Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Itália País de publicação: Suíça