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A comprehensive gene expression analysis of the unique three-layered cocoon of the cecropia moth, Hyalophora cecropia.
Rouhová, Lenka; Podlahová, Sárka; Kmet, Peter; Zurovec, Michal; Sehadová, Hana; Sauman, Ivo.
Afiliação
  • Rouhová L; Biology Centre CAS, Institute of Entomology, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic; University of South Bohemia in Ceske Budejovice, Faculty of Science, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic.
  • Podlahová S; Biology Centre CAS, Institute of Entomology, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic; University of South Bohemia in Ceske Budejovice, Faculty of Science, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic.
  • Kmet P; Biology Centre CAS, Institute of Entomology, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic; University of South Bohemia in Ceske Budejovice, Faculty of Science, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic.
  • Zurovec M; Biology Centre CAS, Institute of Entomology, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic; University of South Bohemia in Ceske Budejovice, Faculty of Science, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic. Electronic address: zurovec@entu.cas.cz.
  • Sehadová H; Biology Centre CAS, Institute of Entomology, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic; University of South Bohemia in Ceske Budejovice, Faculty of Science, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic. Electronic address: sehadova@entu.cas.cz.
  • Sauman I; Biology Centre CAS, Institute of Entomology, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic; University of South Bohemia in Ceske Budejovice, Faculty of Science, Branisovska 31, 370 05, Ceske Budejovice, Czech Republic. Electronic address: sauman@entu.cas.cz.
Insect Biochem Mol Biol ; 171: 104152, 2024 Aug.
Article em En | MEDLINE | ID: mdl-38944399
ABSTRACT
The larvae of the moth Hyalophora cecropia spin silk cocoons with morphologically distinct layers. We investigated the expression of the individual silk protein components of these cocoons in relation to the morphology of the silk gland and its affiliation to the different layers of the cocoon. The study used transcriptomic and proteomic analyses to identify 91 proteins associated with the silk cocoons, 63 of which have a signal peptide indicating their secretory nature. We checked the specificity of their expression in different parts of the SG and the presence of the corresponding protein products in each cocoon layer. Differences were observed among less abundant proteins with unclear functions. The representation of proteins in the inner envelope and intermediate space was similar, except for a higher proportion of probable contaminating proteins, mostly originating from the gut. On the other hand, the outer envelope contains a number of putative enzymes with unclear function. However, the protein most specific to the outer layer has sequence homology to putative serine/threonine kinase-like proteins and some adhesive proteins, and its closest homolog in Bombyx mori was found in the scaffold silk. This research provides valuable insights into the silk production of the cecropia moth, highlighting both similarities and differences to other moth species.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Insetos / Seda / Mariposas Limite: Animals Idioma: En Revista: Insect Biochem Mol Biol Assunto da revista: BIOLOGIA MOLECULAR / BIOQUIMICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: República Tcheca País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Insetos / Seda / Mariposas Limite: Animals Idioma: En Revista: Insect Biochem Mol Biol Assunto da revista: BIOLOGIA MOLECULAR / BIOQUIMICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: República Tcheca País de publicação: Reino Unido