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HumanIslets: An integrated platform for human islet data access and analysis.
Ewald, Jessica D; Lu, Yao; Ellis, Cara E; Worton, Jessica; Kolic, Jelena; Sasaki, Shugo; Zhang, Dahai; Dos Santos, Theodore; Spigelman, Aliya F; Bautista, Austin; Dai, Xiao-Qing; Lyon, James G; Smith, Nancy P; Wong, Jordan M; Rajesh, Varsha; Sun, Han; Sharp, Seth A; Rogalski, Jason C; Moravcova, Renata; Cen, Haoning H; Manning Fox, Jocelyn E; Atlas, Ella; Bruin, Jennifer E; Mulvihill, Erin E; Verchere, C Bruce; Foster, Leonard J; Gloyn, Anna L; Johnson, James D; Pepper, Andrew R; Lynn, Francis C; Xia, Jianguo; MacDonald, Patrick E.
Afiliação
  • Ewald JD; Institute of Parasitology, McGill University, Montreal, QC.
  • Lu Y; Imaging Platform, Broad Institute of MIT and Harvard, Cambridge, MA, USA.
  • Ellis CE; Institute of Parasitology, McGill University, Montreal, QC.
  • Worton J; Alberta Diabetes Institute, University of Alberta, Edmonton, AB.
  • Kolic J; Department of Pharmacology, University of Alberta, Edmonton, AB.
  • Sasaki S; Alberta Diabetes Institute, University of Alberta, Edmonton, AB.
  • Zhang D; Department of Surgery, University of Alberta, Edmonton, AB.
  • Dos Santos T; Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, BC.
  • Spigelman AF; Diabetes Research Group, BC Children's Hospital Research Institute, Vancouver, BC, Canada.
  • Bautista A; Department of Surgery, School of Biomedical Engineering, University of British Columbia, Vancouver, BC.
  • Dai XQ; Diabetes Research Group, BC Children's Hospital Research Institute, Vancouver, BC, Canada.
  • Lyon JG; Department of Surgery, School of Biomedical Engineering, University of British Columbia, Vancouver, BC.
  • Smith NP; Alberta Diabetes Institute, University of Alberta, Edmonton, AB.
  • Wong JM; Department of Pharmacology, University of Alberta, Edmonton, AB.
  • Rajesh V; Alberta Diabetes Institute, University of Alberta, Edmonton, AB.
  • Sun H; Department of Pharmacology, University of Alberta, Edmonton, AB.
  • Sharp SA; Alberta Diabetes Institute, University of Alberta, Edmonton, AB.
  • Rogalski JC; Alberta Diabetes Institute, University of Alberta, Edmonton, AB.
  • Moravcova R; Department of Pharmacology, University of Alberta, Edmonton, AB.
  • Cen HH; Alberta Diabetes Institute, University of Alberta, Edmonton, AB.
  • Manning Fox JE; Alberta Diabetes Institute, University of Alberta, Edmonton, AB.
  • Atlas E; Alberta Diabetes Institute, University of Alberta, Edmonton, AB.
  • Bruin JE; Department of Surgery, University of Alberta, Edmonton, AB.
  • Mulvihill EE; Department of Pediatrics, Division of Endocrinology, Stanford School of Medicine, Stanford, CA.
  • Verchere CB; Stanford Diabetes Research Center, Stanford School of Medicine, Stanford, CA.
  • Foster LJ; Department of Pediatrics, Division of Endocrinology, Stanford School of Medicine, Stanford, CA.
  • Gloyn AL; Stanford Diabetes Research Center, Stanford School of Medicine, Stanford, CA.
  • Johnson JD; Department of Pediatrics, Division of Endocrinology, Stanford School of Medicine, Stanford, CA.
  • Pepper AR; Stanford Diabetes Research Center, Stanford School of Medicine, Stanford, CA.
  • Lynn FC; Department of Biochemistry & Molecular Biology, Michael Smith Laboratories, Life Sciences Institute, University of British Columbia, Vancouver, BC.
  • Xia J; Department of Biochemistry & Molecular Biology, Michael Smith Laboratories, Life Sciences Institute, University of British Columbia, Vancouver, BC.
  • MacDonald PE; Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, BC.
bioRxiv ; 2024 Jun 22.
Article em En | MEDLINE | ID: mdl-38948734
ABSTRACT
Comprehensive molecular and cellular phenotyping of human islets can enable deep mechanistic insights for diabetes research. We established the Human Islet Data Analysis and Sharing (HI-DAS) consortium to advance goals in accessibility, usability, and integration of data from human islets isolated from donors with and without diabetes at the Alberta Diabetes Institute (ADI) IsletCore. Here we introduce HumanIslets.com, an open resource for the research community. This platform, which presently includes data on 547 human islet donors, allows users to access linked datasets describing molecular profiles, islet function and donor phenotypes, and to perform various statistical and functional analyses at the donor, islet and single-cell levels. As an example of the analytic capacity of this resource we show a dissociation between cell culture effects on transcript and protein expression, and an approach to correct for exocrine contamination found in hand-picked islets. Finally, we provide an example workflow and visualization that highlights links between type 2 diabetes status, SERCA3b Ca2+-ATPase levels at the transcript and protein level, insulin secretion and islet cell phenotypes. HumanIslets.com provides a growing and adaptable set of resources and tools to support the metabolism and diabetes research community.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: BioRxiv Ano de publicação: 2024 Tipo de documento: Article País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: BioRxiv Ano de publicação: 2024 Tipo de documento: Article País de publicação: Estados Unidos