Investigation of a sensing approach based on a rapid reduction of azide to selectively measure bioavailability of H<sub>2</sub>S.

Kim, Gunwoo; Jang, Eunju; Page, Alexis M; Ding, Ting; Carlson, Kimberly A; Cao, Haishi

Investigation of a sensing approach based on a rapid reduction of azide to selectively measure bioavailability of H₂S.

Kim, Gunwoo; Jang, Eunju; Page, Alexis M; Ding, Ting; Carlson, Kimberly A; Cao, Haishi.

Afiliação

Kim G; Department of Chemistry, University of Nebraska-Kearney, Kearney, NE, USA 68849.
Jang E; Department of Chemistry, University of Nebraska-Kearney, Kearney, NE, USA 68849.
Page AM; Department of Biology, University of Nebraska-Kearney, Kearney, NE, USA 68849.
Ding T; Department of Chemistry, University of Nebraska-Kearney, Kearney, NE, USA 68849.
Carlson KA; Department of Biology, University of Nebraska-Kearney, Kearney, NE, USA 68849.
Cao H; Department of Chemistry, University of Nebraska-Kearney, Kearney, NE, USA 68849.

RSC Adv ; 6(98): 95920-95924, 2016.

Article em En | MEDLINE | ID: mdl-38988591

ABSTRACT

ABSTRACT

A new reaction-based sensor (AHS) was synthesized for quantitative detection of H2S. AHS showed a high selectivity and sensitivity toward H2S over other thio-containing molecules, or reducing reagents with high abundance in living cells. In the presence of H2S, significant fluorescence enhancement (17-fold) was observed due to the reduction of the azide on AHS. The absorption (362 nm) and fluorescence emission (557 nm) of reduced AHS showed a highly linear correlation to H2S level, which were used to measure concentration of H2S in the range of 0-100 µM.

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: RSC Adv Ano de publicação: 2016 Tipo de documento: Article