Exploring optimal incubation conditions and characteristics of methane oxidizing organisms in deep-sea environments.
Bioresour Technol
; : 131084, 2024 Jul 16.
Article
em En
| MEDLINE
| ID: mdl-39025372
ABSTRACT
Anaerobic methanotrophic archaea (ANME) play key roles in buffering the methane budget in the deep-sea environment. This study aimed to explore the optimal environmental conditions for ANME enrichment. The result showed that the sample at 10.5â¯MPa contained the largest copy numbers of methyl-coenzyme M reductase alpha subunit (mcrA) gene (1.1â¯×â¯106 copies/g) compared to any other pressures and the sample at 4⯰C contained higher mcrA gene (1.6â¯×â¯106 copies/g) than other temperatures. The optimal enrichment pressure for ANME-2c is 10.5â¯MPa at 4⯰C, with an optimal subsequent incubation for ANME-2c less than 211th days. Moreover, the beta nearest taxon index was significantly correlated with the incubation time (P<0.05). Total inorganic carbon and sulfate ion were key environmental factors driving community construction. This study offers insights into how ANME-2c was enriched and how species coexist in shared habitats during enrichment.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Bioresour Technol
Assunto da revista:
ENGENHARIA BIOMEDICA
Ano de publicação:
2024
Tipo de documento:
Article