[Prospective use of immunofluorescence polarization in the study of enzymes modifying aminoglycosides]. / Prospettive di utilizzazione della immunofluorescenza polarizzata per lo studio degli enzimi modificanti gli aminoglicosidi.

ABSTRACT

A number of assays have been described for aminoglycoside-modifying enzymes; the aim of our investigation was to check the validity of polarized immunofluorescence assay for aminoglycoside enzymes. We have studied three enzymes encoded by R-factors from clinical isolates of Enterobacteriaceae conferring various patterns of aminoglycoside-resistance. The assay resulted valid this is a simple, rapid and sensitive method.