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Generating cysteine-trypsin cleavage sites with 2-chloroacetamidine capping.
Ofori, Samuel; Desai, Heta S; Shikwana, Flowreen; Boatner, Lisa M; Dominguez Iii, Emil R; Castellón, José O; Backus, Keriann M.
Afiliação
  • Ofori S; Biological Chemistry Department, David Geffen School of Medicine, UCLA, Los Angeles, CA, 90095, USA. kbackus@mednet.ucla.edu.
  • Desai HS; Biological Chemistry Department, David Geffen School of Medicine, UCLA, Los Angeles, CA, 90095, USA. kbackus@mednet.ucla.edu.
  • Shikwana F; Biological Chemistry Department, David Geffen School of Medicine, UCLA, Los Angeles, CA, 90095, USA. kbackus@mednet.ucla.edu.
  • Boatner LM; Department of Chemistry and Biochemistry, UCLA, CA, 90095, USA.
  • Dominguez Iii ER; Biological Chemistry Department, David Geffen School of Medicine, UCLA, Los Angeles, CA, 90095, USA. kbackus@mednet.ucla.edu.
  • Castellón JO; Department of Chemistry and Biochemistry, UCLA, CA, 90095, USA.
  • Backus KM; Biological Chemistry Department, David Geffen School of Medicine, UCLA, Los Angeles, CA, 90095, USA. kbackus@mednet.ucla.edu.
Chem Commun (Camb) ; 60(67): 8856-8859, 2024 Aug 15.
Article em En | MEDLINE | ID: mdl-39081146
ABSTRACT
An electrophilic arginine mimetic, 2-chloroacetamidine (CAM), was deployed to enable trypsin-mediated proteolysis at cysteine residues and to enhance mass spectrometry-based proteomic detection of cysteine-containing peptides. Illustrating the value of the CAM-capping strategy, proteogenomic analysis using a two-stage false discovery rate (FDR) search revealed >50% enhanced coverage of missense variants, when compared to established workflows.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tripsina / Cisteína Limite: Humans Idioma: En Revista: Chem Commun (Camb) Assunto da revista: QUIMICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tripsina / Cisteína Limite: Humans Idioma: En Revista: Chem Commun (Camb) Assunto da revista: QUIMICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Reino Unido