Protocol for screening and validating antibodies specific to protein phosphorylation sites using a set of yeast biopanning approaches.
STAR Protoc
; 5(3): 103241, 2024 Aug 01.
Article
em En
| MEDLINE
| ID: mdl-39093705
ABSTRACT
Developing antibodies with high specificity against post-translationally modified epitopes remains a challenge. Yeast biopanning is well suited in screening for high-specificity binders. Here, we present a protocol for screening and validating antibodies specific to protein phosphorylation sites using a set of yeast biopanning approaches. We describe steps for screening a yeast surface display library for antibodies and other binders. We then detail procedures for validating the antibodies found by analyzing their specificity through whole-well image analysis in 96-well plates. For complete details on the use and execution of this protocol, please refer to Arbaciauskaite et al.1.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2024
Tipo de documento:
Article