Probing Biological Nitrogen Fixation in Legumes Using Raman Spectroscopy.
Sensors (Basel)
; 24(15)2024 Jul 30.
Article
em En
| MEDLINE
| ID: mdl-39123990
ABSTRACT
Biological nitrogen fixation (BNF) by symbiotic bacteria plays a vital role in sustainable agriculture. However, current quantification methods are often expensive and impractical. This study explores the potential of Raman spectroscopy, a non-invasive technique, for rapid assessment of BNF activity in soybeans. Raman spectra were obtained from soybean plants grown with and without rhizobia bacteria to identify spectral signatures associated with BNF. δN15 isotope ratio mass spectrometry (IRMS) was used to determine actual BNF percentages. Partial least squares regression (PLSR) was employed to develop a model for BNF quantification based on Raman spectra. The model explained 80% of the variation in BNF activity. To enhance the model's specificity for BNF detection regardless of nitrogen availability, a subsequent elastic net (Enet) regularisation strategy was implemented. This approach provided insights into key wavenumbers and biochemicals associated with BNF in soybeans.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glycine max
/
Análise Espectral Raman
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Fixação de Nitrogênio
Idioma:
En
Revista:
Sensors (Basel)
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Nova Zelândia
País de publicação:
Suíça