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Chitosan nanoparticles, Rose Bengal-chitosan activated photodynamic therapy as final irrigant on pushout bond strength, smear layer removal efficacy and antibacterial effectiveness against E.faecalis.
Alkhudhairy, Fahad; AlRefeai, Mohammad H.
Afiliação
  • Alkhudhairy F; Restorative Dental Sciences Department, College of Dentistry, King Saud University, Riyadh, Saudi Arabia. Electronic address: falkhudhairy@ksu.edu.sa.
  • AlRefeai MH; College of Dentistry, Department of Restorative Dentistry - Operative Division. Electronic address: malrefeai@ksu.edu.sa.
Photodiagnosis Photodyn Ther ; 49: 104316, 2024 Oct.
Article em En | MEDLINE | ID: mdl-39181493
ABSTRACT

AIM:

Impact of final root canal disinfectants diode laser (DL), Rose Bengal photosensitizer (RBP), Chitosan Nanoparticles (CNPs), and CNPs modified RBP on Enterococcus faecalis (E.faecalis) survival rate, smear layer (SL) removal efficacy and push out bond strength (PBS) of resin sealer to the radicular dentin. MATERIAL AND

METHOD:

Single rooted seventy-five human mandibular premolar teeth were sectioned at a level of cementoenamel (CEJ) and root canal treatment was performed using a ProTaper rotary instrument. To assess the antibacterial efficiency E.faecalis inoculation was performed using a pour plate method. The samples were arbitrarily allocated into five groups based on the final disinfectant received (n = 15) Group-1 (NaOCl+17 % EDTA), Group-2 (NaOCl+DL), Group-3 (NaOCl+CNPs), Group-4 (NaOCl+RBP activated by DL) and Group-5 (NaOCl+CNPs-RBP activated by DL). SEM analysis for SL removal was performed on five specimens from each group. Root canal obturation and sectioning were performed at cervical, middle, and apical levels. PBS and failure mode were analyzed using a universal testing machine (UTM) and stereomicroscope respectively. The data was subjected to analysis using one-way analysis of variance (ANOVA) and Tukey's post hoc test.

RESULT:

Group 1 (NaOCl+17 % EDTA) (0.36 ± 0.01 CFU/mL) unveiled the maximum survival rate of tested bacteria. However, Group 5 (NaOCl+CNPs-RBP activated by DL) (0.11 ± 0.01 CFU/mL) displayed minimum survival of E.faecalis. The highest SL removal (1.44 ± 0.11) and bond integrity scores (12.23 ± 0.24 MPa) were recorded in the coronal section of Group 5 (NaOCl+ CNPs-RBP activated by DL) treated roots. However, the lowest removal of SL (3.15 ± 1.09) and PBS (7.24 ± 0.09 MPa) were recorded in Group 4 (NaOCl+RBP activated by DL) treated canals.

CONCLUSION:

CNPs-RBP activated by DL as the final irrigant provided satisfactory antibacterial activity, SL removal, and bond strength outcomes compared to other tested groups.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fotoquimioterapia / Rosa Bengala / Enterococcus faecalis / Fármacos Fotossensibilizantes / Quitosana / Nanopartículas Limite: Humans Idioma: En Revista: Photodiagnosis Photodyn Ther Assunto da revista: DIAGNOSTICO POR IMAGEM / TERAPEUTICA Ano de publicação: 2024 Tipo de documento: Article País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fotoquimioterapia / Rosa Bengala / Enterococcus faecalis / Fármacos Fotossensibilizantes / Quitosana / Nanopartículas Limite: Humans Idioma: En Revista: Photodiagnosis Photodyn Ther Assunto da revista: DIAGNOSTICO POR IMAGEM / TERAPEUTICA Ano de publicação: 2024 Tipo de documento: Article País de publicação: Holanda